缺氧性肺动脉高压与血红素氧合酶1及其mRNA变化的关系

The relationship between hypoxic pulmonary hypertension and changes of HO 1 and its mRNA

目的 研究血红素氧合酶１（ Ｈ Ｏ１） 与肺动脉高压（ Ｈ Ｐ Ｈ） 的关系。方法 对正常对照组及缺氧２ 、３ 、５ 周组大鼠，用免疫组织化学技术和组织原位杂交技术观察其肺内 Ｈ Ｏ１ 及其ｍ Ｒ Ｎ Ａ 的分布和含量变化，并用图像分析方法检测肺内３ 种不同管径的动脉中 Ｈ Ｏ１ 的平均积分吸光度值。结果 缺氧诱导了 Ｈ Ｏ１ 及其ｍ Ｒ Ｎ Ａ 的表达且随缺氧时间的延长，表达呈显著性增强。结论 Ｈ Ｐ Ｈ时，肺血管 Ｈ Ｏ１ 表达增强，在 Ｈ Ｐ Ｈ 中起一定作用； Ｈ Ｏ１ ｍ Ｒ Ｎ Ａ 表达水平的变化是 Ｈ Ｏ１ 变化的分子生物学基础。

Objective To study the relationship of HO 1 and HPH Methods The expression of HO 1 and HO 1 mRNA were observed in pulmonary vessles of rats using the technique of immunohistochemistry and hybridization in situ The average value of integral light density of HO 1 in three different diameters of arteries was detected by means of the image analysor and considered as the relative content in pulmonary arteries Results Hypoxia induced the expression of HO 1 and HO 1mRNA and the relative content of HO 1 was significantly increased in pulmonary arteries corresponding with the period of hypoxia Conclusions In the condition of HPH , relative content of HO 1 increase obviously It suggests that HO 1 plays certain role in development of HPH The change of HO 1 mRNA expression is the basis of molecular change of HO 1