AmpliSensor-聚合酶链反应定量检测肺结核患者外周血结核分支杆菌DNA

Quantitative detection of Mycobacterium tuberculosis DNA in peripheral blood from patients with pulmonary tuberculosis by AmpliSensor PCR technique

目的 探讨 Ａｍｐｌｉ Ｓｅｎｓｏｒ聚合酶链反应（ Ａｍｐｌｉ Ｓｅｎｓｏｒ Ｐ Ｃ Ｒ） 定量检测外周血中结核分支杆菌 Ｄ Ｎ Ａ（ Ｔ Ｂ Ｄ Ｎ Ａ） 在肺结核的应用价值。方法 采用 Ｑ Ｉ Ａａｍｐ 和 Ａｃｕ Ｐｕｒｅ 法提取，制备全血中模板 Ｔ Ｂ Ｄ Ｎ Ａ，应用 Ａｍｐｌｉ Ｓｅｎｓｏｒ Ｐ Ｃ Ｒ 定量检测，并与 Ｉ Ｓ６１１０单管巢式聚合酶链反应（ Ｓ Ｎ Ｐ Ｃ Ｒ） 作比较。结果２００ 例肺结核患者的血液标本中，两种方法测得结核分支杆菌 Ｄ Ｎ Ａ 的阳性率分别为６０５ ％ 、６３５ ％ 。８５ 例非结核肺病患者（ 包括２２ 名献血员） 的阳性率分别为４７ ％ 、８２ ％ 。结论 Ａｍｐｌｉ Ｓｅｎｓｏｒ Ｐ Ｃ Ｒ 检测全血中的 Ｔ Ｂ Ｄ Ｎ Ａ 是一种敏感性、特异性较高的方法，其含量的变化对疾病的预后有一定的提示作用。

Objective To evaluate the clinical value of the quantitative detection of Mycobacterium tuberculosis DNA by Amplisensor PCR in peripheral blood from patients with pulmonary tuberculosis Methods The model of Mycobacterium tuberculosis DNA in blood was made by QIAamp and AcuPure methods, quantitative detection of Mycobacterium tuberculosis DNA by AmpliSensor PCR technique and the results were compared with IS6110 single tube nested PCR (SN PCR) Results The peripheral blood from 200 patients with pulmonary tuberculosis were detected respectively by using AmpliSensor PCR and IS6110 SN PCR methods The positive rates of Mycobacterium tuberculosis DNA were 60 5% and 63 5% respectively, and that from 85 patients with non pulmonary tubeculosis were 4 7%, 8 2% Conclusions AmpliSensor PCR method showed higher sensitivity and specificity in detecting the Mycobacterium tubeculosis DNA from peripheral blood The variation of the TB DNA may be useful in evaluating the prognosis of the patients