摘要
为了增强生物方法对秸秆的利用能力,将秸秆较好地转化成氢能,本试验利用改良的Hungate厌氧滚管法及滤纸崩解法,从已得菌系JYB中,筛选得到1株能较好地降解纤维素产氢的细菌,并利用水热法、汽爆法等6种方法对秸秆进行预处理,以增强秸秆的产氢效果。结果表明:筛选得到菌株JYB-13,该菌可利用大量碳源产氢,产氢的最适条件为35℃,pH值8.5。对秸秆进行预处理,由产氢效果得知,该菌对2%硫酸处理后的秸秆降解效果最好,秸秆的降解率为53.1%,氢气含量达到37.2%。分析发酵前后秸秆的成分,可知该菌可以较好地利用纤维素和半纤维素,对木质素利用较少。
In order to strengthen the effect of degradating straw and turn straw to hydrogen,the author used a modified Hungate anaerobic roll tube method and the disintegration of filter paper method,found a strain which could have better hydrogen production by degradating cellulose,and used 6 kinds of method pretreatments,such as hydrothermal method,the vapor on straw stem exploding and so on to treat straw.The author found a bacterium JYB-13 from already getting strains JYB,JYB-13 could produce hydrogen using amount of carbon sources.Its optimum conditions for hydrogen production temperature of 35℃,pH value of 8.5.Using 6 kinds of method pretreatments,by the effect of producing hydrogen,it was concluded that the strain in 2% sulfuric acid treated straw degradation was the best.After fermentation,the degradation rate of straw was 53.1%,the hydrogen content reached 37.2%.Around the analysis of the composition of straw obtained before and after fermentation,JYB-13 could good use of cellulose and hemicellulose better,and few used lignin.
出处
《中国农学通报》
CSCD
北大核心
2011年第3期215-220,共6页
Chinese Agricultural Science Bulletin
基金
863计划重点项目课题(2009AA062906)
关键词
秸秆
纤维素
厌氧
预处理方法
发酵
straw
cellulase
anaerobic
pretreatments
ferment
