多胎与单胎山羊发情期卵巢褪黑素受体基因的差异表达研究

Studies on Differential Display of Melatonin Receptor in Ovary of Gray Goat and Black Goat in Oestrus Stage

为探明褪黑素受体(MTR1A)对山羊产羔数等繁殖性状的调控作用,并比较多胎高产的济宁青山羊与单胎的沂蒙黑山羊发情期卵巢组织中MTR1A的差异表达量。采用荧光定量差异显示PCR(DDRT-qPCR)技术,以持家基因GAPDH为分子内标,对比研究了褪黑素受体1A(MTR1A)基因在多胎的济宁青山羊和单胎的沂蒙黑山羊发情期卵巢组织中的差异表达量。研究发现,MTR1A在两种山羊发情期的卵巢组织中均有表达,但是,在青山羊发情期卵巢组织中的表达量显著低于在沂蒙黑山羊卵巢组织中的表达量(P<0.05)。说明,发情期褪黑素受体基因在卵巢组织中的高表达是限制山羊卵巢排卵和产羔数的重要因素之一。研究结果提示:卵巢组织是褪黑素作用的重要靶器官,褪黑素对于山羊卵巢组织的发育、成熟和排卵具有重要影响。研究结果对于阐明褪黑素受体调控山羊生殖机能的作用机理提供了基础研究数据。

In order to clarify the mechanisms of melatonin affected goat reproduction traits,and compared the different expressed amount of melatonin receptors（MTR1A）in ovary tissues between Jining Grey Goat with high superfoetation and Yimeng Black Goat with single birth.Real-time fluorescent quantitate differential display PCR technique were used to detected the expressed amount of melatonin receptors（MTR1A）in ovary tissure of Jining Gray Goat and Yimeng Black Goat in oestrus stage,while GAPDH internal standards were used as control.The results showed that MTR1A mRNA were expressed in ovary tissue of both goats,and the expressed amount of MTR1A mRNA was lower at Jining Gray Goat ovary than that of it at Yimeng Black Goat ovarys.The results suggested that MTR1A mRNA were expressed in both goat ovary obviously,the differential expression of MTR1A may affect the development,maturity and ovulating of goat ovary.This provided the molecular genetic data to clarity the mechanism of MTR1A action at goat reproduction.