小鼠同源异型盒基因hex的克隆与序列分析

Cloning and Sequence Analysis of the Homeobox hex Gene from KunMing Mouse

同源异型盒基因hex在动物个体发育过程中具有重要的作用。为了解小鼠hex基因在乳腺发育中的作用,研究中提取细胞总RNA,采用RT-PCR方法扩增hex基因,并克隆到pMD18-T载体后测序。结较保守,表明在进化中具有重要的作用。结果表明:小鼠的hex基因开放阅读框由816个核苷酸组成,编码271个氨基酸,相对分子质量为30kD。与已发表的小鼠该基因序列完全一致。与禽类、人和其他动物相比氨基酸序列的同源性在69.8%～97.0%之间。进化树显示,小鼠与大鼠的hex基因在同一分支内,只有7个氨基酸的差异,该蛋白在物种的进化中较保守,标明在进化中具有重要的作用。

Hex（haematopoietically expressed homeobox,hex）gene has important function in vertebrate development.To study the function ofhex gene in mammary gland development,the total RNA was extracted,hex gene was amplified by RT-PCR and cloned into pMD18-T in the study.The results indicated that the open reading frame ofhex gene was 816 bp in length which could encode 271 amino acids,was 30 kD molecular weight.There was complete similarity with the sequence published from GenBank.The homology of amino acids were 69.8%-97.0% with chicken,human,rat,xenopus and zebrafish.The phylogenetic tree of Hex protein from different species proved that there were seven differences in amino acid sequence between mouse and rat.HEX protein was conserved in sequence,it had important function in evolution.