摘要
利用马齿苋的叶片诱导出愈伤组织,再以愈伤组织为受体建立了根癌农杆菌介导的遗传转化体系。利用该转化体系得到抗性愈伤组织后,对其进行GUS组织化学染色和PCR扩增鉴定,证实为转化子,表明根癌农杆菌介导外源基因转化马齿苋的愈伤组织是完全可行的,为以后通过基因工程手段进行马齿苋的改良奠定了基础。
The callus was induced from the leaves of Portulaca oleracea L.,and the callus was used for the receptor of genetic transformation mediated by Agrobacterium tumefaciens.The resistant callus was screened and confirmed to be transformants by GUS histochemical assays and PCR.It showed that genetic transformation of Portulaca oleracea L.callus mediated by Agrobacterium tumefaciens was entirely feasible.
出处
《中国农学通报》
CSCD
北大核心
2011年第4期139-143,共5页
Chinese Agricultural Science Bulletin
基金
农业部公益性行业科研专项资金资助项目"核技术农业应用"(200803034)
关键词
马齿苋
愈伤组织
根癌农杆菌
遗传转化
Portulaca oleracea L.
callus
Agrobacterium tumefaciens
genetic transformation
