树莓品种‘Kivigold’组培快繁技术体系建立

Tissue Culture and Rapid Propagation of Raspberry(Rubus idaeus)Cultivar‘Kivigold’

以树莓(Rubus idaeus)品种‘Kivigold’带腋芽茎段为外植体,研究消毒剂HgCl(20.1%)不同的消毒时间对外植体培养的影响,对不定芽增殖培养基和生根培养基中适宜的激素种类及浓度、生根苗移栽驯化中适宜的基质进行了筛选。结果显示,树莓品种‘Kivigold’外植体的最佳消毒时间为10min,初代培养时只需添加6-BA即可满足外植体萌发和生长的需要;在不定芽增殖过程中,细胞分裂素主要影响不定芽的增殖,而生长素主要影响不定芽的生长,以质量浓度低于1.5mg/L的6-BA以及质量浓度低于0.5mg/L的NAA较为适宜,3种碳源中蔗糖更有利于不定芽的增殖和生长;经过进一步的筛选,确定适宜于‘Kivigold’不定芽增殖和生长的培养基为MS+1.00mg/L6-BA+0.10mg/LNAA(含20g/L蔗糖和5.9g/L琼脂,pH5.8),适宜于‘Kivigold’不定芽生根的培养基为1/2MS+0.10mg/LNAA(含20g/L蔗糖和5.9g/L琼脂,pH5.8);在移栽驯化中最适宜的栽培基质为泥炭土:珍珠岩=1:1,移栽成活率可达到93.33%。

The sterilization time of HgCl2（0.1%）in initial culture,suitable type and concentration of phytohormone in proliferation and rooting culture media of adventitious bud,suitable matrix in transplant were selected using the stem segment with an axillary bud of raspberry（Rubus idaeus）cultivar‘Kivigold’as explants,and also suitable sugar during proliferation culture process was studied.The results showed that ininitial culture,the optimal sterilization time of 0.1%HgCl2was 10 min,the explants could grow well on MSmedium with only 6-BA;The single factor experiment revealed that cytokinin mainly affects on proliferation while auxin affects on growth during proliferation process of adventitious bud,and medium with 6-BA concentration below 1.5 mg/L and NAA concentration below 0.5mg/L was more appropriate;Sucrose was more beneficial to bud growth during proliferation process of adventitious bud;Through a further combinative selection,it was determined that the medium suitable for prolife ration o‘fKivigold’adventitious bud was MS＋1.00 mg/L 6-BA＋0.10 mg/L NAA（containing 20 g/L sucrose and 5.9 g/L agar,pH 5.8）,the optimum medium for rooting o‘fKivigold’adventitious bud was 1/2 MS＋0.10 mg/L NAA（containing 20 g/L sucrose and 5.9 g/Lagar,pH 5.8）;Peat soil＋perlite at the rate of 1：1 was the suitable matrix for transplanting,the survival rate was 93.33%.