井冈霉素·枯草芽孢杆菌对小麦纹枯病菌室内毒力测定及药效试验

Toxicity Test and Effectivity of the Mixture of Validamycin and Bacillus subtilis to Rhizoctonia cerealis

为了明确5%井冈霉素WP与200亿孢子/g枯草芽孢杆菌WP复配对小麦纹枯病的毒力作用,通过菌丝生长速率法测定了二者不同配比对小麦纹枯病菌的室内毒力,根据Wadley法评价增效作用,并对筛选出的最佳配比的药剂进行了盆栽药效实验,结果表明:5%井冈霉素WP与200亿孢子/g枯草芽孢杆菌WP在1:1的配比时增效系数最大,增效最为显著,此配比制剂用量500、125、250mg/L时的预防效果分别达到78.8%,77.3%,75.1%,均高于50%多菌灵WP(167mg/L)和5%井冈霉素WP(250mg/L),治疗效果和预防效果明显。5%井岗霉素WP与200亿孢子/g枯草芽孢杆菌1:1复配可作为防治小麦纹枯病的理想药剂。

In order to clear the toxicity of the mixture of validamycin 5% WP and Bacillus subtilis （200 hundreds million spores per gram） to Rhizoctonia cerealis;the toxicity test was determined in laboratory by mycelium growth rate method.According to Wadley method,the synergistic activity was evaluated,and pot experiment of different ratio was studied.Results showed that coefficient of synergism was the highest and synergistic activity was the strongest when the ratio of validamycin 5% WP and Bacillus subtilis （200 hundreds million spores per gram） was 1：1.It ＇ s preventing effect were 78.8%,77.3%,75.1% when concentration was 500,125,250 mg/L respectively,higher than carbendazim 50% WP （167 mg/L） and validamycin 5% WP （500 mg/L）.The preventing effect and cure effect were evident.The mixture of validamycin 5% WP and Bacillus subtilis （200 hundreds million spores per gram） could taken as an ideal fungicides to Rhizoctonia cerealis.