颐和园古桑树的组织培养与快速繁殖

In vitro Propagation of Ancient Mulberry (Morus alba) from the Summer Palace

以北京颐和园古桑树为外植体进行组织培养,取桑树当年新生嫩茎切段为外植体进行,其中不定芽启动培养基为MS+6-BA0.5mg/L+NAA0.1mg/L+PVP0.2g/L;将无菌苗接到最适分化培养基MS+6-BA0.8mg/L+NAA0.1mg/L+PVP0.2g/L上,组培苗分化率高;不定根最适诱导培养基为:1/2MS+NAA0.5mg/L+PVP0.2g/L,生根率达100%。组培苗移栽成活率达95%。

Nodal explants derived from ancient mulberry （Morus alba ）,which was lived in the Summer Palace for more than 100 years,were used to initiate shoot cultures.Bud formation on the nodal explants was induced on an optimal medium MS＋6-BA 0.5 mg/L＋NAA 0.1 mg/L＋PVP 0.2 g/L.To get a high propagation ratio,the seedlings were transferred to the medium of MS＋ 6-BA 0.8 mg/L ＋NAA 0.1 mg/L ＋PVP 0.2 g/L.The optimal medium for adventitious root inducing was 1/2MS＋NAA 0.5 mg/L ＋PVP 0.2 g/L,and the rooting rate was 100%.The plantlets were transplanted to pots with vermiculite-perlite mixture-humus soil （1：1：1） mixture and acclimated for several weeks.The rooted and acclimated plantlets were transferred outdoors with 95% transplantation success.