高温木质素降解菌Geobacillus caldoxylosilyticus J16的筛选及其产酶发酵性质研究

Isolation of Lignin Degraded Thermophilic Geobacillus Caldoxylosilyticus Strain J16 and Characteristics of its Enzymes and Fermentation

从张家界、白云山采集到的朽木及落叶中,经分离、纯化后,获得了一株高温木质素降解菌,命名为地芽孢杆菌(Geobacillus caldoxylosilyticus J16),此菌嗜热,耐高温,只降解木质素且不降解纤维素,对造纸业和可再生能源产业具有重要意义。笔者通过对木质素中2种酶木质素过氧化物酶、锰过氧化物酶活性的研究,确定了木质素过氧化物酶的最适温度为65℃,最适pH为4,在55℃至70℃之间时酶活力较其他温度高且较稳定;锰过氧化物酶最是温度为60℃,最适pH为3在温度为55℃至65℃之间时,活力较其他温度高。作者发酵了黄豆杆、玉米杆、芝麻杆、油菜杆、锯末、小麦杆这6种农业废弃物,通过发酵前后的比较,说明了J16菌株对木质素降解的最大减少量为7.5%。上述数据显示此菌可以应用于废弃秸秆的处理和造纸厂污水的处理,对环保有重要意义.

The thermophilic Geobacillus caldoxylosilyticus strain J16 was isolated after screening many microorganisms.Its factors was thermophilic,but could not degrade cellulose.It was significance for paper industry and renewable energy industry.The factors were studied,which effected on lignin peroxidase and manganese peroxidase.The strain could grow very well at 65℃ and pH 4.0.When fermented with the agriculture wastes about soybean stem,maize stem,rape stem,sawdust,wheat stem and sesame stem,it could efficiently degrade lignin up to 7.5%,but not degrade cellulose.All of above showed that it was significant for paper industry and renewable energy industry.All the data showed that this strain could be applied to agriculture wastes and sewage,and it was very important for environmental protection.