脆性组氨酸三联体基因与子宫颈癌生物学行为的相关性

Clinical significance of FHIT gene and its relationship with clinicopathologicai feature in cervicalcarcinoma

目的 探讨脆性组氨酸三联体（FHIT）基因蛋白与子宫颈癌生物学行为的相关性。方法采用免疫组织化学sP法对20例慢性子宫颈炎和95例ⅠA～ⅢB期子宫颈癌放疗前、后患者进行FHIT蛋白表达水平的检测，分析FHIT蛋白表达水平与子宫颈癌临床病理特征之间的关系。结果子宫颈癌组织中FHIT蛋白表达降低与子宫颈癌组织学分级、组织类型、淋巴结转移、浸润深度有关（X2值分别为10．761、10．853、5．602，P〈0．05）；而与年龄、临床分期无关（P〉0．05）。子宫颈癌患者放疗前FHIT蛋白表达阳性率为36．0％（18／50），明显低于放疗后的84．0％（42／50）（X2=2．271，P〈0．05），且表达阳性率与放疗剂量呈正相关（X2=6．791，P〈0．01）。放疗前后FHIT蛋白阳性表达者3年生存率均高于阴性表达者（93．75％比41．27％，70．83％比25．00％），差异有统计学意义[放疗前X2=28．729，P〈0．05；放疗后P〈0．05（精确慨率法）]。结论放疗前后FHIT蛋白表达水平对评估子宫颈癌的生物学行为和预后有重要的临床参考价值：

Objective To investigate the expression fragile histidinetriad （FHIT） protein in cervical carcinoma and its relationship with clinicopathological feature of the disease. Methods Immunohistochemistry SP was used to detect the expression of FHIT protein in 20 cases with chronic cervsis and 95 cases with Ⅰ a- Ⅲ b stage cervical carcinoma before and after treatment. The association of the expression of FHIT with clinicopathological feature was analyzed by the statistical method. Results There were significant differences between FHIT expression and histological grades and types of tissue, lymph node metastasis and invade depth （P 〈0.05）. FHIT expression was not correlated with age and clinical stage （P 〉0.05）. There were significant differences in FHIT protein expression levels in the patients with cervial cancer between befure-after radiotherapy and the levels before radiotherapy was lower then those after radiotherapy （P 〈0.05）. There was positively correlated in FHIT protein expression rates before and after radiotherapy （P 〈0.05）. There were significant correlation between FHIT expression and 3-year survival rate, the positive rates of the expression FHIT protein higher then negative ones （P 〈0.05）. Conclusion FHIT protein has great reference value that could be as a parameter for evaluating biological action and predicting the prognosis of cervical cancer.