摘要
目的 研究负载有肺癌细胞株A549可溶性抗原并携带外源粒细胞巨噬细胞集落刺激囚子（GM—CSF）基因的树突状细胞（DC）在体外激活自身T淋巴细胞形成的细胞毒T淋巴细胞（CTI．）对A549细胞的杀伤作用：方法用肺癌细胞株A549可溶性抗原致敏DC，再用含有GM—CSF外源基凶的腺病毒感染DC，将所得的DC与T细胞混合培养以形成对A549细胞有特异杀伤作用的CTL。细胞分为未处理（N—DC）组、加抗原未感染病毒（A—DC）组和加抗原感染病毒（G—A—DC）组。通过测定乳酸脱氢酶（LDH）计算CTL对A549细胞的杀伤率。结果当CTL与A549细胞，即效应细胞与靶细胞比值（E／T）为5：1时，N—DC组的杀伤率为（1．9±0．7）％，A—DC组为（21．3±2．6）％，G—A—DC组为（34．5±4．9）％；当E／T为10：l时，N—DC组的杀伤率为（4．8±0．8）％，u—DC组为（35．4±3．6）％，G—A—DC组为（51．3±2．9）％；E／T为20：1时，N，DC组的杀伤率为（5．3--．t-O．2）％，A—DC组为（40．5±7．7）％，C—A—DC组为（72．5±4．7）％。3组之间的杀伤率差异有统计学意义（n=2，F：356，P〈0．05），通过两两比较，得出（j—A—DC组的杀伤率高于其他两组（P〈0．001），且A—DC组高于对照组（P〈0．001）。结论以帅癌细胞株A549可溶性抗原致敏的DC可诱导卅对A549具有特异杀伤作用的CTL，当所致敏的DC通过腺病毒感染而带有外源基因GM—CSF时，所诱导的细胞毒杀伤反应进一步增强。
Objective The goal of the study was to assess the lethal effect of cytotoxic lymphocyte against A549 cells induced by dendritic cells （DC） pulsed with A549 lysate and transfected with GM-CSF recombinant adenovirus. Methods The cytotoxic lymphocyte against A549 cells were induced by culturing with DCs, which pulsed with A549 antigens and transfected with GM-CSF recombinant adenovirus. The cytotoxic lymphocyte against A549 cells responses were measured by LDH release detection. Results At 5：1 of effector/target ratio, the killing rates of N-DC group, A-DC group and G-A-DC group were （1.9±0.7） %, （21.3±2.6） % and （34.5±4.9） %, respectively. At 10：1 of effector/target ratio, the killing rates of N-DC group, a-DC group and G-A-DC group were （4.8±0.8） %, （35.4±3.6） % and （51.3±2.9） %, respectively. At 20：1 of effector/target ratio, the killing rates of N-DC group, A-DC group and G-A-DC group were （5.3±0.2） %, （40.5±7.7） % and （72.5±4.7） %, respectively＇. We found that the killing rate of G-A-DC group was the highest by statistics. Conclusion The cytotoxic lymphocyte against A549 cells can be induced by DCs pulsed with A549 lysate ,and the lethal effect of CTLs can be enhanced when DCs were infected with GM-CSF recombingnant adenovirus.
出处
《肿瘤研究与临床》
CAS
2011年第3期201-203,共3页
Cancer Research and Clinic
