摘要
以加拿大紫荆‘森林火焰’(Cercis canadensis‘Forest Pansy’)当年生嫩枝为试验材料,探讨不同培养基和植物生长调节剂如6-BA、IBA、NAA、TDZ对试管苗增殖以及生根的影响。结果表明:最适增殖培养基为DKW+TDZ0.03mg/L+PVP0.5mg/L;生根培养基为1/2MS+IBA25mg/L+AC0.6mg/L。此体系中增殖系数可达4.27,生根率73.3%。
Young stem sections with buds ofCercis canadensis‘Forest Pansy’were used as explants for experiment. The effects of culture mediums and PGRs such as 6-BA,NAA,IBA,TDZ on proliferation and rooting of test-tube plantlets were discussed. The results showed that the medium suitable for proliferation was DKW+TDZ 0.03 mg/L+PVP 0.5 mg/L,the optimum rooting medium was 1/2 MS+IBA 25 mg/L+AC 0.6 mg/L. In this system,the highest propagation coefficient was 4.27 and the rooting rate was 73.3%.
出处
《中国农学通报》
CSCD
北大核心
2011年第6期64-67,共4页
Chinese Agricultural Science Bulletin
基金
山东省农业良种产业化项目"优良园林绿化彩叶树种引进筛选"
关键词
加拿大紫荆'森林火焰’
组织培养
增殖
生根
Cercis canadensis‘Forest Pansy’
tissue culture
proliferation
rooting