东方百合试管鳞茎形成条件优化

Culture Condition Optimization of Test Tube-bulb Formation of Lily-Orientals

试管鳞茎培养是百合种球培育的关键技术环节。为探讨不同种类植物生长调节剂和不同浓度蔗糖对东方百合试管鳞茎形成的影响,试验采用正交设计,筛选出最优诱导培养基为MS+0.2mg/L KT+1mg/L NAA+40g/L蔗糖;最优膨大培养基为1/2MS+0.2mg/L KT+1mg/L NAA+15mg/L PP333,此条件下膨大率可达到80%以上;最优生根培养基为1/2MS+0.05mg/L NAA。

Test-tube bulblet culture is the key technology during the cultivation of lily bulb. To find out the effects of plant growth regulators and sucrose in different concentration on the test-tube bulblet formation,orthogonal test was designed. The result showed that the optimal medium treatment for bulblets induction was MS＋0.2 mg/L KT＋ 1 mg/L NAA＋40 g/L sugar,and the optimal medium for bulb enlargement was 1/2 MS＋ 0.2 mg/L KT＋1 mg/L NAA＋ 15 mg/L PP333,under which condition the swelling rate was more than 80%,and the optimal medium for rooting was 1/2 MS＋0.05 mg/L NAA.