凝胶渗透色谱净化烟叶有机磷、拟除虫菊酯类农药残留的研究

Study on GPC Clean-up of Residues of Organophosphorous Pesticides and Synthetic Pyrethroid Pesticides in Tobacco Leaf

为确定凝胶渗透色谱（GPC）净化烟草有机磷和拟除虫菊酯农药残留的最佳收集时间,提高烟草有机磷和拟除虫菊酯农药残留的净化效果和回收率,进行了此项试验。采用添加3个浓度梯度农药标准品进行回收试验的方法,研究了烟叶中9种有机磷农药和7种拟除虫菊酯农药在GPC柱上的流出规律。结果表明,有机磷农药的最佳收集时间为26-68min,拟除虫菊酯类农药的最佳收集时间为26-42min。有机磷农药的回收率为80.28%-96.81%,相对标准偏差为4.44%-8.74%,拟除虫菊酯类农药回收率为76.39%-97.95%,相对标准偏差为2.69%-7.58%。GPC净化烟草有机磷和拟除虫菊酯农药残留的最佳收集时间与国家标准GB/T13595不同,主要是因为农药品种不同而造成的,因此最佳收集时间必须根据具体试验来确定。

In order to determine the optimum collecting time of GPC clean-up organophosphorous and pyrethroid residue in tobacco, and improve of purificatory effect and recoveries of those pesticide residues, the test was in progress. According to recovery tests for 16 organophosphorous and pyrethroid pesticide standard spiked samples with 3 concentration gradients, elution behavior of from a gel permeation chromatography column （GPC） was determined for these pesticides in tobacco leaf. The results showed that the optimum collection time of 9 organophosphorous pesticides from GPC column was 26-68 min and that for 7 pyrethroid pesticides was 26-42 min. The recoveries of organophosphorous pesticides were 80.28%-96.81%, the relative standard deviation were 4.44%-8.74% , the recoveries of pyrethroid pesticides were 76.39%-97.95% , the relative standard deviation were 2.69%-7.58% . The optimum collecting time of GPC clean-up pesticide residue is different from GB/T 13595, so the optimum collecting time must be determined by test.