IBA、NAA和蔗糖浓度对东北刺人参不定根增殖生长的影响

Effects of IBA, NAA and Sucrose Concentration on Adventitious Root Growth of Oplpanax elatus Nakai in Vitro

【研究目的】为大量扩繁东北刺人参不定根提供可靠的理论依据。【方法】以东北刺人参不定根为外植体,研究在液体振荡培养中,NAA、IBA以及蔗糖浓度对东北刺人参不定根生长的影响。【结果】不同生长素及浓度对东北刺人参不定根的影响中,IBA对促进东北刺人参不定根的生长效果优于NAA,当IBA浓度为3~4mg/L时,适合不定根生长,培养不定根的IBA浓度为3mg/L;在培养基MS＋IBA3mg/L中提高蔗糖浓度到50g/L时,可明显促进东北刺人参不定根的鲜物重和干物重的增加,分别为5.3、0.49g。【结论】培养基配方为MS＋IBA3mg/L＋50g/L蔗糖且振荡培养时,东北刺人参不定根的生长效果最好。

[ OBJECTIVE ] The aim was to provide the theoretical basis for large scale propagation adventitious root of Oplpanax elatus Nakai. [ METHOD ] With the adventitious root of Oplpanax elatus Nakai as explants, the effect of concentrations of IBA, NAA and sucrose on adventitious root growth of Oplpanax elatus during suspension culture was examined in this study. [ RESULTS ] Auxin types and concentration affected adventitious roots growth, roots weights of fresh and dry were better in the medium supplanted with IBA than with NAA, IBA concentration of 3 or 4 mg/L was suitable for roots growth. Sucrose was also a important factor for adventitious roots growth, roots weights of fresh and dry were promoted by 50 g/L sucrose, and the values reached 5.3, 0.49 g respectively. [ CONCLUSION ] Adventitious root of Oplpanax elatus Nakai grew better when the formula of medium was MS＋IBA 3 mg/L＋50 g/L sucrose in suspension culture.