摘要
为了优化马齿苋多糖的提取工艺,将纤维素酶用于提取马齿苋多糖,在单因素实验的基础上,采用L9(34)正交实验法对酶法提取马齿苋多糖的条件进行优化,考察酶用量、酶解温度、pH、酶解时间对马齿苋多糖提取得率的影响。结果表明:最合适的工艺参数为酶用量2.0%、酶解温度40℃、pH6.0、酶解时间110min。该条件下,马齿苋多糖得率达到9.7%。酶法提取纤维素多糖工艺简洁,能量消耗减少,是一种提取马齿苋多糖的的适宜方法。
In order to extraction of polysaccharide from Portulaca oleracea L.. The optimum enzyme extraction technology was optimized with L 9 （3 4 ） orthogonal test. The influence of enzyme dosage,extraction temperature , pH and extraction time on yield of polysaccharide was studied. The optimum conditions were as follows： dosage of cellulose 2.0%, temperature 40℃, time 110 min, pH 6.0 and extraction yield were 9.7%. It indicated that enzyme extraction was a suitable method for extraction polysaccharide from Portulaca oleracea L. for it was simple and has shorter extraction time.
出处
《中国农学通报》
CSCD
北大核心
2011年第5期475-478,共4页
Chinese Agricultural Science Bulletin
关键词
酶
提取
马齿苋
多糖
enzyme
extraction
Portulaca oleracea L.
polysaccharide
