枇杷叶总黄酮超高压提取及抗氧化活性

Ultra High Pressure Extraction and Antioxidant Activities of Total Flavonoids from Eriobotrya japonica (Thumb.) Lindl

为研究超高压提取的枇杷叶总黄酮的抗氧化能力,选用L9(34)正交实验对枇杷叶总黄酮的提取工艺条件进行优选,以总黄酮获得率为指标,考察乙醇浓度、超高压压力、超高压提取时间、固液比g/mL对总黄酮获得率的影响,并且测定了总黄酮对DPPH自由基的清除作用。结果表明:总黄酮提取最佳工艺条件为:乙醇浓度50%、超高压压力400MPa、超高压提取时间4.5min、固液比1:18。该条件下总黄酮的获得率可达0.693%,总黄酮浓度和对DPPH自由基的清除作用呈现线性相关。超高压提取的总黄酮具有较强的抗氧化能力,具有较高的应用价值。

In order to study the antioxidant activities of ultra high pressure （UHP） extractiontotal flavonoids from Eriobotrya japonica （Thumb.） Lindl.. The influence of ethanol concentration, UHP pressure, UHP time and the ratio of solid to liquid on total flavonoids yield were studied by using. L 9 （3 4 ） orthogonal design. Meanwhile, the free radical scavenging of DPPH was determined .The results shown that the optimum extraction conditions were ethanol concentration 50%, UHP pressure 400 MPa, UHP time 4.5 min, stock ratio 1：18 g/mL.The yield of total flavonoids was 0.693% .The results also shown that line relationship existed between DPPH free radical scavenging and total flavonoids. The UHP extraction total flavonoids have better higher antioxidant activities and has great exploiting foreground.