采后青花菜花球抗氧化水平变化与衰老的关系

Correlation Between Antioxidative Level and Senescence of Broccoli Florets During Postharvest Storage

为研究采后青花菜花球抗氧化水平变化与衰老的关系,将采后青花菜花球贮藏于20℃条件下,连续4 d测定了花蕾衰老生理指标(叶绿素、蛋白质、丙二醛(MDA)含量)及抗氧化水平指标(抗坏血酸(AsA)和β-胡萝卜素(β-Car)含量、超氧化物岐化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)和抗坏血酸过氧化氢酶(APX)活性)。结果表明:贮藏过程中,叶绿素和蛋白质含量逐渐减少,MDA含量逐渐增多;AsA和β-Car含量逐渐减少,SOD、CAT和POD活性逐渐升高,APX活性逐渐降低;这些抗氧化水平指标与衰老生理指标的线性相关性均在1%水平显著。说明采后青花菜花球的抗氧化水平变化与衰老密切相关。

In this work, relationship between the senescence of broccoli （Brassica oleracea L. Var. italica） florets and their changes in antioxidative level was investigated. Postharvest broccoli florets were stored at 20℃ , and its indicators of senescence （ chlorophyll, protein and malondialdehyde （MDA） contents） and antioxidative level （ascorbic acid and 13-carotene contents, superoxide dismutase （SOD）, catalase （CAT） peroxidase （POD） and ascorbate peroxidase （APX） activities） of flower buds were analyzed in continuous 4 days during the storage. With increasing of the storage time, chlorophyll and protein contents decreased, MDA content increased, ascorbic acid and β-carotene contents decreased, SOD, CAT and POD activities increased and APX activity decreased. These antioxidative level indicators showed linear correlation to senescence indicators at P = 0.01. These results showed that senescence of postharvest broccoli florets is closely correlated to antioxidative level.