黄芪对血吸虫发育及感染宿主免疫应答的影响

Effect of astragalus on schistosome development and host immune response

目的:了解黄芪对日本血吸虫(Sj)生长发育与其感染小鼠免疫应答的影响。方法:取黄芪注射液,在体内用0.25g/d和0.5 g/d两个剂量分别给Sj感染昆明鼠饮服50 d后,观察小鼠体内Sj生长发育情况,并检测实验小鼠血清中抗卵和抗成虫抗体、IL-6和IL-10水平。结果:对Sj的影响:体内试验结果,与感染对照组比,低量黄芪组、高量黄芪组、低量黄芪+半量吡喹酮(PZQ)以及半量PZQ组的减虫率分别为29.2%、30.3%、43.2%和35.8%,雌虫体长度减少率分别为10.49%、10.57%、33.61%和22.76%,雄虫体长度减少率分别为8.40%、15.33%、32.5%和28.52%,肝卵减少率分别为31.1%、36.0%、47.4%和46.9%。对宿主免疫应答的影响,与感染对照组比,两黄芪组鼠血清中抗SEA抗体水平和IL-6水平降低均显著降低(P<0.05),IL-10水平显著增高(P<0.05)。结论:黄芪对Sj童虫具有一定抗虫作用,并对感染小鼠可下调抗卵抗体、促炎因子的应答水平,上调抗炎因子表达。

AIM：To understand the influence of astragalus on the development of Schistosoma japonicum（Sj） and immune response of mice infected with Sj.METHODS：Kunming strain of mice were naturally and succes-sively administrated astragalus for 50 d to observe its influence on Sj-infected mice and detect the level of antibody against Sj adoult worm antigens（SjAWA） and level of Sj soluble egg antigens（SjSEA）,IL-6,and IL-10 immune response to hosts infected by Sj.RESULTS：The results showed that compared with infected controls,after admin-istration with low-dose astragalus（0.25 g/d）,high-dose astragalus（0.5 g/d）,low-dose astragalus ＋ praziquantel and praziquantel group,respectively,the reduction rates of worm were 29.2%,30.3%,43.2% and 35.8%,re-spectively,and the body lengths of females reduced 10.49%,10.57%,33.61% and 22.76% and that of males 8.40%,15.33%,32.50% and 28.52% and liver eggs reduced 31.1%,36.0%,47.4% and 46.9%.The lev-els of antibody against SEA,IL-6 and a pro-inflammatory cytokine in the four administrated groups but for infected group were significantly reduced（either P 〈0.05 or P 〈0.01）,but the level of IL-10,an anti-inflammatory cyto-kine was risen（P 〈0.05）.CONCLUSION：Astragalus plays a role in anti-growth and development of Sj and im-mune response to antibody against eggs,down-regulates proinflammatory factor and up-regulates antiinflammatory factor.