RP-HPLC测定脑络通胶囊中原儿茶醛、阿魏酸、丹参酮Ⅱ_A和盐酸托哌酮的含量

Determination of protocatechuic aldehyde,ferulic acid,tanshinone Ⅱ_A and tropisetron hydrochloride risperidone in Naoluotong Capsule by RP-HPLC

目的:建立测定脑络通胶囊(丹参浸膏、盐酸托哌酮、川芎浸膏等)中原儿茶醛、阿魏酸、丹参酮ⅡA和盐酸托哌酮含量的RP-HPLC法。方法:采用ODS C18柱为固定相,甲醇-1%冰醋酸(30∶70)、甲醇-水(75∶25)、甲醇-0.3%冰醋酸(25∶75)为流动相,检测波长321 nm、270 nm、261 nm。结果:原儿茶醛、阿魏酸、丹参酮ⅡA和盐酸托哌酮的线性范围分别为0.008～0.08 mg/mL(r=0.999 99),0.008～0.08 mg/mL(r=0.999 99),0.004～0.04 mg/mL(r=0.999 96),0.050～0.5mg/mL(r=0.999 98);平均回收率分别为98.11%(RSD=0.73%);98.66%(RSD=0.83%);98.65%(RSD=0.48%);99.15%(RSD=0.57%)。结论:本方法简便快速,结果准确可靠,适用于脑络通胶囊的质量控制。

AIM：To establish the method for determining protocatechuic aldehyde,ferulic acid,tanshinone ⅡA and tropisetron hydrochloride ris peridone in Naoluotong Capsule（Extract of Radix et Rhizoma Salviae miltior-rhizae,tropisetron hydrochloride risperidone,extract of Rhizoma Chuanxiong,etc.） by HPLC.METHODS：The Hypersil-ODS C18 column（250 mm ×4.6 mm,5 μm）was used and detection wavelengths were at 321 nm、270 nm and 261 nm.The mobile phases were methanol-1% glacial acetic acid（30 ∶ 70）,methanol-water（75 ∶ 25）,methanol-0.3% glacial acetic acid（25 ∶ 75）.The flow rate was 1.0 mL/min.RESULTS： The calibration curves of protocatechuic aldehyde,ferulicacid,tanshinone ⅡA and tropisetron hydrochloride risperidone were in a good linearity over the ranges of 0.008-0.08 mg/mL（r = 0.999 99）,0.008-0.08 mg/mL（r = 0.999 99）,0.004-0.04 mg/mL（r =0.999 96）,0.050-0.5 mg/mL（r =0.999 98） respectively.The average recoveries and RSD（n =9） were 98.11%（RSD =0.73%）;98.66%（RSD =0.83%）;98.65%（RSD =0.48%）;99.15%（RSD =0.57%）respectively.CONCLUSION： The method is simple,rapid,reliable and can be used for the drug control.