摘要
目的:采用液质联用技术(LC-MS)对中药柔肝胶囊(丹参、姜黄等)中的化学成分进行定性分析。方法:运用AC-QUITY UPLC HSS T3(2.1 mm×100 mm,1.8μm)柱,以甲醇-0.1%甲酸为流动相梯度洗脱;质谱使用ESI离子源,正离子与负离子模式下采集数据。结果:通过Q-TOF/MS正、负离子质谱信息及元素组成分析并结合对照品与相关文献数据对照,共鉴定出19个化合物,主要结构类型为:水溶性酚酸类、姜黄素类、皂苷类及异黄酮类化合物。结论:本法对柔肝胶囊的成分鉴定提供一种快速准确的分析方法。
AIM:To analyze and identify the chemical components in Rougan Capsule(Radix et Rhizoma Salvi-ae miltiorrhizae,Rhizoma Curcumae longae,etc.) by Ultra Performance Liquid Chromatography-Electrospray Ioni-zation-Quadrupole Time of Flight/Mass Spectrometry(UPLC-ESI-QTOF/MS).METHODS:These compounds were analyzed with an ACQUITY UPLC HSS T3(2.1 mm × 100 mm,1.8 μm) by gradient elution using methanol and 0.1% formic acid.ESI source was used and data were acquired in positive and negative mode.RESULTS:Nineteen compounds in Rougan Capsules were identified by direct comparison in both positive and negative modes of QTOF/MS,the elemental compositions analysis,the standard substance and the literature values,the main structural type were composed of waters soluble phenols,curcumin constituents,saponins,and isoflavones.CONCLUSION:It is a rapid and accurate method for tracing to source of compounds in Rougan Capsules.
出处
《中成药》
CAS
CSCD
北大核心
2011年第1期73-78,共6页
Chinese Traditional Patent Medicine