黄连生物碱的HPLC-MS分析

Analysis of the main alkaloids of Coptis chinensis by HPLC-MS

目的:利用高效液相色谱-质谱法,对黄连药材中的化学成分进行定性研究并对主要成分进行定量研究。方法:采用Waters Symmetry C18(3.9 mm×150 mm,5μm)色谱柱;流动相为乙腈和0.05%甲酸水梯度洗脱;检测波长345 nm;流速为0.8 mL/min;柱温35℃;正离子检测模式;电喷雾离子化(ESI)。结果:鉴定了8个成分分别为木兰花碱、格陵兰黄连碱、黄连碱、表小檗碱、非洲防己碱、药根碱、小檗碱和巴马汀;同时对小檗碱、药根碱、黄连碱和巴马汀进行了含量测定。结论:该方法简便、准确,可用于黄连药材的质量评价。

AIM：To develop a method for qualitatively and quantitatively analysing the constituents in Coptis chinensis by HPLC with diode-array detection and mass-spectrometer.METHODS：The chromatographic separa-tion was performed on a Waters Symmetry C18 column（5 μm,3.9 mm × 150 mm） using acetonitrile and 0.05% formic acid aqueous solution under gradient program at a flow rate of 0.8 mL/min.Column temperature was 35 °C,detection wavelength was set at 345 nm with finnigan ion trap mass spectrometer and positive ion mode.RESULTS：Eight characteristic compounds were identified as magnoflorine,groenlandicine,coptisine epiberberine,columbamine,jatrorrhizine,berberine and palmatine by HPLC-DAD-MS.Based on the qualitation of eight compounds,four compounds were further quantified.CONCLUSION： This improved method provides a feasible and comprehensive technique for the quality control of Coptis chinensis Franch.