摘要
目的:建立急性子药材中黄酮类成分的HPLC指纹图谱。方法:采用Diamonsil TM C18柱(250 mm×4.6mm,5μm),以甲醇-0.4%磷酸水溶液为流动相梯度洗脱,流速为1.0 mL/min,检测波长为365 nm,柱温30℃。结果:建立了急性子中黄酮类成分的指纹图谱,标定出12个共有峰;对11个不同产地急性子药材的指纹图谱进行相似性比较,相似度达到0.85以上。结论:该方法重现性好,可行性强,可用于急性子药材的质量控制。
AIM：To establish an HPLC fingerprint of flavonoids in Impatientis Semen.METHODS： HPLC separation was performed on a Diamonsil TM C18 analytical column with methanol-0.4% phosphoric acid aqueous being as mobile phase in gradient elution mode at the flow rate of 1.0 mL/min.The UV detection wavelength was set at 365 nm.The column temperature was 30 ℃.RESULTS： Twelve mutual peaks were fixed in the HPLC fin-gerprint of flavonoids from Impatientis Semen.The similarities of eleven peaks from different habitats were more than 0.85.CONCLUSION： This method shows good reproducibility and better feasibility,and can be used as the means to assess the quality of Impatientis Semen.
出处
《中成药》
CAS
CSCD
北大核心
2011年第2期199-202,共4页
Chinese Traditional Patent Medicine
关键词
急性子
黄酮
HPLC指纹图谱
Impatientis Semen
flavonoids
HPLC fingerprint
