摘要
目的:建立急性子药材中黄酮类成分的HPLC指纹图谱。方法:采用Diamonsil TM C18柱(250 mm×4.6mm,5μm),以甲醇-0.4%磷酸水溶液为流动相梯度洗脱,流速为1.0 mL/min,检测波长为365 nm,柱温30℃。结果:建立了急性子中黄酮类成分的指纹图谱,标定出12个共有峰;对11个不同产地急性子药材的指纹图谱进行相似性比较,相似度达到0.85以上。结论:该方法重现性好,可行性强,可用于急性子药材的质量控制。
AIM:To establish an HPLC fingerprint of flavonoids in Impatientis Semen.METHODS: HPLC separation was performed on a Diamonsil TM C18 analytical column with methanol-0.4% phosphoric acid aqueous being as mobile phase in gradient elution mode at the flow rate of 1.0 mL/min.The UV detection wavelength was set at 365 nm.The column temperature was 30 ℃.RESULTS: Twelve mutual peaks were fixed in the HPLC fin-gerprint of flavonoids from Impatientis Semen.The similarities of eleven peaks from different habitats were more than 0.85.CONCLUSION: This method shows good reproducibility and better feasibility,and can be used as the means to assess the quality of Impatientis Semen.
出处
《中成药》
CAS
CSCD
北大核心
2011年第2期199-202,共4页
Chinese Traditional Patent Medicine
关键词
急性子
黄酮
HPLC指纹图谱
Impatientis Semen
flavonoids
HPLC fingerprint