甘草饮片乙酸乙酯提取部位HPLC指纹图谱研究

Fingerprint of ethyl acetate extract of Glycyrrhizae Radix et Rhizoma Praeparta

目的:建立甘草饮片乙酸乙酯提取部位高效液相指纹图谱,比较生炙饮片乙酸乙酯部位化学成分差异。方法:采用反向高效液相色谱法,选用Hyperclone ODS C18柱(4.6 mm×250 mm,5μm),乙腈-0.2%冰醋酸作为流动相梯度洗脱,柱温:30℃;流速:1 mL/min;检测波长:310 nm。时间:60 min。结果:10批甘草测定显示:炙甘草乙酸乙酯提取物检测到8个共有峰,少于生甘草乙酸乙酯提取物2个共有峰,蜜炙使甘草极性成分峰面积增大。结论:该方法稳定、准确、可靠,符合指纹图谱技术要求。研究结果可为阐明甘草蜜炙后增强补脾益气药效作用的物质基础,从化学成分转化的角度为解析甘草蜜炙原理提供依据。

AIM：To establish an HPLC fingerprint of ethyl acetate extract of Glycyrrhizae Radix et Rhizoma praeparata cum melle（honey liquorice-root） and compare with fingerprint of non-processing liquorice-root.METH-ODS： RP-HPLC was used with Hyperlone ODS C18 column（4.6 mm ×250 mm,5 μm）,mobile phase consisted of acetonitrile-0.2% acetic acid in gradient elution mode,detection wavelength was set up at 310 nm,and the flow rate was 1.0 mL/min.RESULTS： From ten batches of honey liquorice-root,ethyl acetate extracts originated from stir with honey had eight common peaks,and was two less than ethyl acetate extracts originated from non-process-ing.Stir-with honey processing made peak of liquorice-root polar constituents enlarged.CONCLUSION： The method is stable,accurate,and the methodological study shows that results meet the technical requirements for fin-gerprint.Stir-with noney can increase the liquorice-root of tonifying spleen nourishing qi.