野菊花水煎剂中7种药效组分的含量测定

Determination of the seven active components in water extraction of Chrysanthemi Indici Flos

目的:建立同时测定野菊花中黄酮类及有机酸类药效组分含量的方法。方法:以12个产地的野菊花水煎剂为载体,采用HPLC法测定。Diamonsil C18色谱柱(4.6 mm×250 mm,5μm);流动相:A相为0.5%磷酸水溶液,B相为乙腈,进行梯度洗脱,在0～10 min,B相从10%线性改变至20%,10～30 min,B相从20%线性改变至30%,30～40 min,B相从30%线性改变至40%;检测波长为326 nm;柱温:35℃。结果:绿原酸、咖啡酸、3,5-二咖啡酰奎尼酸、木犀草素-7-O-β-D-葡萄糖苷、秋英苷、蒙花苷、木犀草素的线性范围分别为0.122 4～2.448μg(r=0.999 84)、0.027 2～0.544μg(r=0.999 88)、0.295 2～5.904μg(r=0.999 91)、0.056 4～1.128μg(r=0.999 90)、0.043～0.86μg(r=0.999 72)、0.262～5.24μg(r=0.999 91)和0.022～0.44μg(r=0.999 81)。回收率分别为98.07%、98.73%、98.12%、98.59%、98.24%、98.75%和98.31%,RSD分别为1.96%、0.73%、1.01%、1.14%、1.10%、1.02%和1.00%(n=5)。结论:该方法同时测定7种有效成分,准确、快速、重现性好,适合于测定野菊花中有机酸类及黄酮类药效组分的含量。

AIM：To develop an HPLC method for determining the active components of flavonoids and organic acids in water extraction of Chrysanthemi Indici Flos.METHODS： Chrysanthemi Indici Flos from twelve habitats as carrier and HPLC was used.The chromatographic separation was performed on a Diamonsil C18（4.6 mm × 250 mm,5 μm） eluted with a mobile phase of 0.5% H3PO4-acetonitrile,in the first 10 min,acetonitrile changed from 10% to 20% linealy,and in the next 20 min,where acetonitrile changed from 20% to 30%,and in the last 10 min,where acetonitrile changed to 40%.The detection wavelength was set at 326 nm and column temperature was set at 35 ℃.RESULTS： Nice linear relation between the peak area and sample size existed when the amount was within 0.122 4-2.448 μg（r = 0.999 84） for chlorogenic acid;0.027 2-0.544 μg（r = 0.999 88） for caffeic acid;0.295 2-5.904 μg（r = 0.999 91） for 3,5-O-dicaffeoylquinic acid;0.056 4-1.128 μg（r = 0.999 90） for luteolin-7-O-glucoside;0.043-0.86 μg（r = 0.999 72） for apigenin-7-O-β-D-glucoside 0.262-5.24 μg（r = 0.999 91） for buddleoside and 0.022-0.44 μg（r = 0.999 81） for luteolin.The recoveries were 98.07% 、 98.73% 、98.12% 、98.59% 、98.24% 、98.75% and 98.31%,respectively,and RSD were 1.96% 、0.73% 、 1.01% 、1.14% 、1.10% 、1.02% and 1.00%（n = 5）,respectively.CONCLUSION： The method is accurate,reproducible and suitable to determination of the seven active components in Chrysanthemi Indici Flos instantane-ously.It can be used for quality control of Chrysanthemi Indici Flos.