白芍对川楝子减毒作用机制研究

Attenuation of Fructus Toosendan with Radix Paeoniae Alba

目的:通过配伍中药白芍考察对川楝子的减毒效应及其机制,以探求减轻川楝子肝毒性的中药复方减毒配伍规律。方法:SD大鼠,分组,单次灌服蒸馏水或川楝子、川楝子+白芍,放射免疫法检测肿瘤坏死因子(TNF-α)、白细胞介素-6(IL-6)含量、免疫组化法检测核转录因子-κB(NF-κB)、ICAM-1蛋白表达。Western-blot法检测caspase-3、bcl-2基因表达。结果:白芍与川楝子配伍应用后,能够对抗肝组织TNF-α、IL-6水平的提高(P<0.05或P<0.01),能对抗肝组织NF-κB、ICAM-1蛋白表达的增强(P<0.01);能够调节肝组织caspase-3、bcl-2的基因表达。结论:白芍能对抗川楝子导致的肝损伤,其减毒机制是该药可以减轻肝组织炎症反应,并与调节肝细胞坏死相关基因caspase-3、Bcl-2的表达有关。

AIM：To find the way to reduce the hepatic toxicity of Fructus Toosendan by the use of Radix Paeoniae Alba METHODS：SD rats were assigned to three experimental groups：control group（given distilled water）,Fructus Toosendan group and Fructus Toosendan ＋ Radix Paeoniae Alba group.The contents of tumor necrosis factor-alpha（TNF-α） and interleukin-6（IL-6） were determined by ELISA;The protein expression of nuclear transcription factor-κB（NF-κB） and intercellular adhesion molecule-1（ICAM-1） were detected by immunohistochemistry;The expression of caspase-3 and bcl-2 were detected by Western-blot.RESULTS：After adding Radix Paeoniae Alba to Fructus Toosendan,it could antagonize the enhancement of TNF-α and IL-6 level in the rats＇hepatic tissue and the protein expression of NF-κB and ICAM-1 significantly（P0.05 or P0.01）,also could be againstthe enhancement of the genetic expression of caspase-3 and the decrease of the genetic expression of bcl-2.CONCLUSION：Radix Paeoniae Alba can lower the hepatic injury induced by Fructus Toosendan via lessening inflam-matory reaction in relation to caspase-3 and bcl-2.