摘要
对胀袋西式火腿中的优势腐败菌进行分离,并通过16S rRNA基因序列分析对分离菌株进行鉴定,综合分析确定各菌株分别为枯草芽孢杆菌、短小芽孢杆菌与生孢梭状芽孢杆菌;以分离菌株为目标菌,进行蜂胶抑菌实验。结果表明:蜂胶对分离菌株及菌株混合液均有明显抑菌效果,并确定最低抑菌浓度为:枯草芽孢杆菌(0.1g/100mL)、短小芽孢杆菌(0.2g/100mL)、菌株混合液(0.2g/100mL)、生孢梭状杆菌(0.3g/100mL)。
The dominant bacteria in spoiled ham were isolated and identified based on 16S rRNA sequence analysis. They were identified as Bacillus subtilis, Bacilluspumilus, and Clostridium sporogenes. Antibacterial tests of propolis on the strains isolated were carded out, and the results showed that propolis had obvious inhibitory effects on single strains and their mixture. Minimal inhibitory concentration （MIC） was determined as 0.1 g/100 mL for B. subtilis, 0.2 g/100 mL for B. pumilus, 0.2 g/100 mL for their mixture, and 0.3 g/100 mL for C. sporogenes.
出处
《肉类研究》
2011年第2期5-8,共4页
Meat Research
关键词
肉制品
腐败菌
PCR
蜂胶
meat products
spoilage bacteria
PCR
propolis
