新疆假龙胆中有效成分提取工艺的研究

Studies of extraction process for effective component in Gentianella turkestanorum Gard. Holub scabra Bge

目的：优选龙胆的最佳提取工艺。方法：比较冷浸提取、超声提取、加热回流提取、渗漉提取和煎煮提取等5种提取方式。采用L9（3^4）正交试验，以龙胆苦苷、龙胆总苷的提出率和干膏得率为考察指标，通过多指标综合评分法进行数据分析，对影响龙胆提取工艺的因素进行了研究。结果：龙胆的最佳提取工艺为：龙胆药材用70％乙醇徊热回流提取3次，每次1h．溶剂用量分别为10倍、8倍、8倍。结论：优选得到的提取工艺稳定可行，适合工业化生产。

Objective： To optimize the extraction process for effective component from [ Gentianella turkestanorum （Gard. Holub] scabra Bge. Methods： The extraction process was investigated by soaking, transonic, hot refluxing, percolation and decoction u.ith water. The orthogonal test design L9 （34） was employed to optimized the extraction condition, and the synthetic score based on the extraction yield of gentiopicrin, total glycosides and total solid was used as the indice. Results： The optimum extraction process was： refluxing and extracting the morcel materials for 3 times（ 1 hour each time） with 10 - fold 70% ethanol added in the first time and then 8 - fold in the second and the third time. Conclusion：The optimized extraction procedure is stable and simple, it is suitable for industrial production.