放射性^(125)I粒子植入对裸鼠人乳腺癌细胞移植瘤的抗肿瘤作用

Anti-tumor effects of ^(125)I radioactive particles implantation on transplantated tumor model of human breast cancer cells in nude mice

目的:探讨放射性125I粒子植入对裸鼠人乳腺癌细胞种植瘤的抗肿瘤作用,阐明其抗肿瘤机制。方法:将120只人乳腺癌细胞MCF-7移植瘤模型的裸鼠,随机分为3组:放射性125I粒子植入组、空粒子植入组和无粒子植入组,每组40只,按照巴黎系统原则植入粒子。采用RT-PCR和Western blotting法检测肿瘤组织中Fas mRNA和蛋白的表达以及caspase-3和caspase-8酶的活性,流式细胞术检测细胞周期。结果:人乳腺癌细胞MCF-7放射性125I植入组与空粒子组及无粒子植入组比较,肿瘤组织体积明显缩小(P<0.05),FasmRNA、Fas蛋白、caspase-3及caspase-8表达均明显增高(P<0.05),其中caspase-3和caspase-8表达均大于0.6。流式细胞术显示,放射性125I粒子植入组G0/G1期细胞显著增多(P<0.05),而S期细胞明显降低(P<0.05)。结论:放射性125I粒子植入人乳腺癌肿瘤内可以杀伤肿瘤细胞,抑制肿瘤细胞生长周期,促进肿瘤细胞凋亡。

Objective To study the anti-tumor effects of 125I radioactive particles implantation on transplantated tumor model of human breast cancer cells in nude mice and clarify their anti-tumor mechanisms.Methods 120 nude mice transplantated with human breast cancer cells MCF-7 were randomly devided into 3 groups（n=40）：125I radioactive particles implanted group,non-radioactive particles implanted group and non-particles implanted group.The articles were implanted into mice according to Pairs system principle.The expressions of Fas mRNA and protein and the activaties of caspase-3 and caspase-8 enzyme were detected by RT-PCR and Western blotting.The changes of cell cycle were detected by flow cytometry.Results Compared with non-radioactive particles implanted group and non-particles implanted group,the size of cancer tisses in 125I radioactive particles implanted group was reduced significantly（P〈0.05）.The expressions of Fas mRNA,Fas protein,caspase-3 and caspase-8 were incresed remarkably（P〈0.05）.The expressions of caspase-3 and caspase-8 were more than 0.6.The flow cytometry results showed that the number of cells in G0/G1 phase was significatly increased（P〈0.05） while the number of cells in S phase was decreased remarkably（P〈0.05）.Conclusion The implantation of125I radioactive particles into transplantated tumor model of human breast cancer cells can kill tumor cells,inhibit the growth cycle of tumor cells and induce the apoptosis of tumor cells in nude mice.