细胞黏附分子SLeX和CD24在喉鳞状细胞癌组织中的表达

Expressions of SLeX and CD24 in laryngeal squamous cell carcinoma tissues

目的:探讨细胞黏附分子唾液酸化Lewis-X抗原(SLeX)和CD24在喉鳞状细胞癌组织中的表达,阐明其在喉鳞状细胞癌发生发展中的作用。方法:采用免疫组织化学方法分别检测42例喉鳞状细胞癌患者的42个原发灶、24个相应癌旁组织及20例睡眠呼吸暂停低通气综合征(OSAHS)手术患者的咽部正常黏膜组织中SLeX和CD24的表达情况。结果:喉癌组织、癌旁组织及对照正常黏膜组织中SLeX阳性表达率分别为71.4%、37.5%和30.0%;强阳性表达率分别为50.0%、8.3%和5.0%。CD24的阳性表达率分别为64.3%、33.3%和25.0%;强阳性表达率分别为35.7%、4.2%和0.0%。3组间两两比较,喉癌组织与癌旁组织、正常黏膜组织阳性表达率差异均有统计学意义(P<0.01);癌旁组织与正常黏膜组织阳性表达率差异无统计学意义(P>0.05)。SLeX、CD24强阳性表达与颈部淋巴结转移情况、临床分期及组织病理学分级相关(P<0.05)。SleX和CD24在喉癌组织中的阳性率呈显著正相关关系(r=0.695,P<0.05)。结论:SLeX及CD24蛋白表达与喉鳞状细胞癌的发生发展密切相关,其检测对喉癌的生物治疗和预测预后具有一定的临床意义。

Objective To discuss the expressions of SLeX and CD24 in laryngeal squamaous cell carcinoma（LSCC） tissues and clarify their roles in the occurrence and development in of LSCC.Methods The expressions of SLeX and CD24 in 42 cases of LSCC tissues,24 cases of adjacent tissues,and 20 cases of normal mucosa tissues were detected by immunohistochemical method.Results The positive expression rates of SLeX in laryngeal cancer tissues group,adjacent tissues group and normal tissues group were 71.4%,37.5%,and 30.0%,respectively.The positive expression rates of CD24 in laryngeal cancer tissues group,adjacent tissues group and normal tissues group were 64.3%,33.3%,and 25.0%,respectively.The strong positive expression rates of SLeX in laryngeal cancer tissues group,adjacent tissues group and normal tissues group were 50.0%,8.3%,and 5.0%,respectively.The strong positive expression rates of CD24 in laryngeal cancer tissues group,adjacent tissues group and normal tissues group were 35.7%,4.2%,and 0.0%,respectively.The positive expression rates of SLeX and CD24 had significant differences among laryngeal cancer tissues,adjacent tissues and normal tissues（P〈0.01）.There was no significant difference between adjacent tissues and normal tissues（P〉0.05）.The strong positive expressions of SLeX and CD24 had significant correlation with the metastases of the cervical lymph nodes,the clinic stages and histopathological classification.The expression level of SLeX had positive correlation with CD24 in LSCC tissue（r=0.695,P〈0.05）.Conclusion The expressions of SLeX and CD24 are related to the occurrence and development of LSCC.The detection of the expressions of SLeX and CD24 has clinical significance on biotherapy and prognosis of LSCC.