DPP Ⅳ基因影响卵巢癌细胞生物学行为的机制探讨

Mechanisms Investigation of Biological Behavior of Ovarian Carcinoma Cell lines Influenced by Gene DPP Ⅳ

目的探讨DPPⅣ基因高表达对卵巢癌细胞系恶性行为的影响及其机制。方法应用体外增殖能力、黏附和侵袭试验检验HO-8910PM、HOPcDNA和HODPP Ⅳ细胞恶性行为差异;利用流式细胞技术、吖啶橙染色、DNA ladder、透射电镜以及Western blot检测Fas/Bcl-2基因蛋白表达水平,探讨HO-8910PM、HOPcDNA和HODPP Ⅳ细胞中凋亡状况。结果 DPPⅣ基因高表达可显著抑制卵巢癌细胞的增殖、黏附和侵袭能力;同时HODPP Ⅳ细胞的凋亡率、Fas基因蛋白表达水平明显高于HOPcDNA和HO-8910PM细胞(P<0.05);HO-8910PM和HOPcDNA细胞的Bcl-2基因蛋白表达水平明显高于HODPP Ⅳ细胞(P<0.05)。结论 DPPⅣ基因高表达可抑制卵巢癌细胞的恶性行为;凋亡可能是DPPⅣ基因发挥生物学作用机制之一。

Objective To investigate the influence of gene DPP IV high expression on the malignant behaviour of ovarian carcinoma cell lines and its mechanisms.Methods The various malignant behaviour of cell lines HO-8910PM,HOPcDNA and HODPP Ⅳ was detected by test of in vitro proliferative,adhesive and invasive activity.To investigate the apoptosis of cell lines HO-8910PM,HOPcDNA and HODPP Ⅳ,the flow cytometry,acridine orange dyeing,DNA ladder and transmission electron microscope were applicated.Meanwhile,the protein expression of gene Fas/Bcl-2 was detected by Western blot.Results The high expression of gene DPP IV significantly depressed proliferative,adhesive and invasive activity of ovarian carcinoma cells.The apoptosis rate and protein expression of gene Fas of cell line HODPP Ⅳ were higher than those of cell lines HO-8910PM,HOPcDNA（P0.05）;while the protein expression of gene Bcl-2 of cell lines HO-8910PM,HOPcDNA was higher than cell line HODPP Ⅳ（P0.05）.Conclusion The high expression of gene DPP IV can depress the malignant behavior of ovarian carcinoma cell lines.Apoptosis might be one of the mechanisms of gene DPP Ⅳeffect.