摘要
以禽肠炎沙门菌基因组DNA为模板,采用PCR技术扩增得到外膜蛋白OMPX基因片段,并将其克隆到乳酸乳球菌表达载体pMG36e中,构建重组质粒pMG36e-OMPX。将重组质粒电转入乳酸乳球菌MG1614,得到重组基因工程乳酸乳球菌,在GM17培养基中培养12h后,经SDS-PAGE分析显示表达的蛋白约为16 000,与预期相符,经Western-blot检测表明,表达的蛋白具有良好的反应特异性。
To clone and express the gene encoded the outer membrane protein X of Salmonella enteritidis.The PCR product was cloned into pMG36e vector,recombinant expression plasmid pMG36e-OMPX was constructed.Then the recombinants were transferred into the host strain MG1614,after cultured 12 hours in GM17 medium,SDS-PAGE showed a 16 000 protein was expressed and Western-blot confirmed the antigenicity against positive sera.The recombinant protein could be useful resource for development of genetic engineering Lactobacillus.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2011年第4期489-492,共4页
Chinese Journal of Veterinary Science
基金
国家现代农业产业技术体系建设专项资金资助
山东省科技支撑计划资助项目(2007GG20009001)
国家"十一五"科技支撑计划重大项目(2006BAK02A21)
关键词
沙门菌
外膜蛋白
乳酸乳球菌
表达
Salmonella enteritidis
outer membrane protein X
Lactococcus lactis
expression
