不同时期旋毛虫抗原对肥大细胞的作用

Differential activation of mast cells by antigens fromTrichinella spiralis muscle larvae,adults warm and newborn larvae

观察旋毛虫不同时期抗原对肥大细胞释放组胺和β-氨基己糖苷酶的影响。用质量浓度为1、5、25、50、100μg/L的肌幼虫抗原、成虫抗原、新生幼虫抗原分别与2.5×104的肥大细胞37℃条件下作用10min。采用荧光检测系统检测在上清液面及下层颗粒组分中的组胺水平,计算组胺释放率;加入20μL相同质量浓度的旋毛虫不同时期抗原分别与50μL 5mol/L对硝基酚-N-乙酰-β-D-葡糖胺和肥大细胞37℃孵育2h,酶标仪测定D值,计算β-氨基己糖苷酶释放率。结果显示,25μg/L肌幼虫抗原刺激肥大细胞释放组胺率最高,50μg/L最低;成虫抗原50μg/L最高,1μg/L最低,但均高于阴性对照组(P<0.05);而新生幼虫刺激肥大细胞不释放组胺,旋毛虫各期抗原不刺激肥大细胞释放β-氨基己糖苷酶。结果表明,肥大细胞在抗寄生虫感染的最初阶段扮演重要角色。

To observe the effect of Trichinellas piralis antigens on mast cells to release histamine andβ-hexosaminidase at different stages.muscle larvae antigen,adult antigen,newborn larvae antigen at concentration of 1,5,25, 50and 100μg/L were cultivated with mast cells（2.5×10 4）for 10min at 37℃.The fluorescence detection system was used to detect histamine level in the supernatant fractions and lower level of particle fractions,and the histamine release rate was calculated.20Lof Trichinellas piralis antigens at the same concentration and stages as above and 50 L 5mol/L of PNP-N-acetyl-β-D-glucosamine were cultivated with mast cells for 2hat 37℃,microplate reader was used to measure Dvalue and release rate ofβ-hexosaminidase was calculated.The release rate of histamine of mast cell stimulated by muscle larvae antigen at concentration of 20μg/L showed the highest value,while the group stimulated by muscle larvae antigen with concentration of 50μg/L indicated the lowest value;in addition,as for groups stimulated by adult antigen,a concentration of 50μg/L showed the highest release rate of histamine,1μg/L displayed the lowest value,but both of them were higher than the negative control group.The group of mast cell stimulated by newborn larvae did not release histamine, while Trichinella spiralis antigens at any stages did not stimulate mast cells to releaseβ-hexosaminidase.Mast cells plays an important role in the initial stage of anti-parasite infection.