苏丹红Ⅰ号的毛细管电泳安培检测

Determinnation of Sudan I by capillary electrophoresis with amperometric detection

建立了毛细管电泳安培法测定苏丹红Ⅰ号的方法。考察了缓冲液种类、浓度和pH值,分离电压、进样时间、检测电位等因素对分离检测的影响。以10 mmol/L硼砂盐（pH 9.3）为缓冲液,检测电位1.05 V（vs.SCE）,分离电压21 kV,电动进样（12 kV）8 s,苏丹红Ⅰ号在1.13×10-9~1.13×10-7mol/L范围内,浓度与峰面积具有良好的线性关系,回收率为93.5%~102.7%,检测限为1.13×10-9mol/L。该方法成功应用于辣椒粉样品的检测,结果令人满意。

A method based capillary with amperometric detection was developed for the determinnation of Sudan dye（Ⅰ）.The buffer pH value,the types of buffer solution,the concentration of the buffer,the applied voltage and the sampling time were selected as the investigation variables.Sudan Ⅰ was achieved using a background electrolyte consisting of 10 mM borate（pH 9.3）.Detection potential was ＋1.05 V（vs.SCE）.Injection time was 8 s（12 kV）.Excellent linearity was obtained in the concentration rang from 1.13×10-9~1.13×10-7 mol/L for Sudan Ⅰand recoveries were 93.5%~102.7%.The limit of detection was 1.13×10-9 mol/L（S/N3）.This method has been successfully applied for chilli powder samples,with satisfactory results.