静电纺丝素/聚丁二酸丁二醇酯血管材料的结构与性能

Structure and properties of electrospun silk fibroin-poly(butylene succinate) artificial blood vessel

为制备组织工程血管支架,以丝素蛋白(SF)和聚丁二酸丁二醇酯(PBS)为原料,通过静电纺丝法,以具有三维结构的收集模板取代传统的二维平板作为静电纺丝收集基板,构建丝素/PBS血管支架材料。研究纺丝条件和三维收集模板对管状支架形貌的影响,采用FT-IR对丝素蛋白二级结构进行表征,测试血管支架材料的孔隙率和力学性能。结果表明,通过改变电压、纺丝间距以及三维收集模板的宏观结构,可制备出具有不同直径和长度的血管支架材料。这种支架材料的孔隙率达84.6%,拉伸应力为4.31 MPa,断裂伸长率为46.21%,爆破压力为358 kPa。

To develop a small-diameter tissue engineering blood vessel,the silk fibroin（SF）/ poly（butylene succinate）（PBS） tubular scaffolds were fabricated via electrospinning.During the electrospinning process,the electrospun SF/PBS composite tubular scaffolds were collected on a 3-D collector instead of traditional 2-D collector.The effects of 3-D collector and process parameters of electrospinning on the morphologies of the tubular scaffolds were investigated.FT-IR was used to study the secondary structure of SF.The mechanical properties and porosities of the tubular fiber materials were characterized.The results showed that SF/PBS composite tubular materials with different sizes and shapes could be prepared by changing the voltage,TCD and the structure of 3-D collectors.The porosity,tensile stress,elongation at break and burst pressure of the composite tubular scaffold were 84.6%,4.31 MPa,46.21% and 358 kPa,respectively.