梓醇对D-半乳糖致亚急性衰老小鼠学习记忆及脑中相关抗氧化酶活性的影响

Effects of catalpol on memory and antioxidative enzyme activity in D-galactose induced sub-acute senescent mice

目的观察梓醇对D-半乳糖(D-gal)致亚急性衰老小鼠学习记忆及脑组织中相关抗氧化酶活性的影响。方法用D-gal皮下注射制备衰老小鼠模型,同时在前两周和后两周分别皮下注射给予梓醇(2.5 mg/kg),检测小鼠学习记忆能力和大脑皮层、海马中丙二醛(MDA)水平,超氧化物岐化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)活性。结果衰老模型组小鼠空间学习记忆能力下降,逃避潜伏期较正常对照组明显延长,脑中SOD、GSH-PX活性降低,MDA含量增高。给予梓醇预防和治疗后可以改善衰老模型小鼠的学习记忆能力,并显著提高脑中SOD、GSH-PX活性,降低MDA水平。结论梓醇可改善D-gal致衰老小鼠的学习记忆障碍,此作用可能与梓醇的抗氧化作用有关。

Purpose To investigate the effects of catalpol on the cognition ability and anti-oxidative ability of senescent mice induced by D-galactose.Methods Except control group,mice were subcutaneously injected with D-galactose（150 mg/kg body weight）for 6 weeks.Meanwhile,drug precaution and treatment group mice were treated with catalpol（2.5 mg/kg body weight）for the first and last 2 weeks.Spatial learning and memory ability was examined using Morris water maze test after drug administration.The activities of superoxide dismutase（SOD）,glutathione peroxidase（GSH-PX）and the level of malondialdehyde（MDA）in mice brain were determined using biochemical method.Results The results showed that injection of D-galactose caused not only degression of spacial cognition ability,but also damage of anti-oxidative ability in the brain of senescent mice.Administration of catalpol for two weeks reversd the D-galactose induced behavioral impairments,increased the activities of SOD,GSH-PX and decreased the MDA level in the cerebral cortex and hippocampus of D-galactose treated mouse.Conclusion All these suggested that the antiaging effects of catalpol were partly mediated via enhancing endogenous antioxidant enzymatic activities and alleviating lipid peroxidation in the brain of senescent mice.