摘要
目的观察壳寡糖对内毒素刺激的小鼠巨噬细胞(RAW 264.7)的影响。方法采用半定量RT-PCR检测IL-1β的基因水平变化,ELISA分析蛋白水平变化,流式细胞仪分析细胞凋亡情况。结果壳寡糖可显著抑制内毒素介导的炎症细胞因子IL-1β的增高,0.4 mg/mL的壳寡糖抑制效果最为明显,并显著降低内毒素介导的细胞凋亡。结论壳寡糖可显著保护内毒素介导的细胞损伤。
Purpose To investigate the effects of chitooligosaccharides on LPS-stimulated RAW 264.7 cells.Methods Interleukin-1β（IL-1β） mRNA expression was measured by semi-quantitative RT-PCR and ELISA analysis of IL-1β production showed the similar results,cells apoptosis was detected by FACS analysis.Results Chitooligos accharides significantly reduced the production of pro-inflammatory mediator IL-1β,especially at the concentration of 0.4 mg/mL.Furthermore,we found that COS inhibited cell apoptosis in LPS-stimulated RAW 264.7 cells.Conclusion Therefore,chitooligosaccharides could protect cell injury from LPS stimulation.
出处
《中国生化药物杂志》
CAS
CSCD
北大核心
2011年第2期136-138,共3页
Chinese Journal of Biochemical Pharmaceutics
关键词
壳寡糖
内毒素
细胞凋亡
chitooligosaccharides
lipopolysaccharides
cell apoptosis
