4种油脂对瘤胃原虫群体和纤维降解菌的影响

Effects of Different Oils on Rumen Protozoa Population and Cellulolytic Bacteria in vitro

试验旨在研究不同油脂对体外培养瘤胃原虫群体结构、纤维降解菌及纤维素降解性能的影响。以3头瘤胃瘘管山羊为瘤胃液供体,分对照、菜籽油(A)、豆油(B)、玉米油(C)、花生油(D)5组体外培养。结果表明:各组间原虫密度差异显著(P<0.05),A到D组有依次上升的趋势;内毛虫、等毛虫、双毛虫、前毛虫等类群原虫组间变化显著(P<0.05)。4种油脂对产琥珀酸拟杆菌、白色瘤胃球菌、生黄瘤胃球菌的影响不大,B组溶纤丁弧菌显著高于对照组(P<0.05)。A组的4种纤维降解菌基本都显著低于对照组与其他试验组(P<0.05)。总之,添加4%的油脂除菜籽油外对纤维降解性能没有明显的负面影响,均对微生物的群体结构有不同程度的影响。

The main goal of the experiment was to compare the effects of different oils on rumen microbial population and cellulose degradability in vitro.Three goats fitted with cannulas were used to provide rumen liquor;And 5 groups were control group,rapeseed oil（A）,soybean oil（B）,corn oil（C）,peanut oil（D）,respectively.The results showed that,no significant difference was detected in protozoal protein among groups,while remarkable differences were found in protozoal densities across groups,and protozoal densities tended to increase from A to D.Protozoal community structures differ from each other,and significant differences were found in several genuses,such as Entodinium,Isotrichidae,Epidinium,Diplodiniinae across groups.In addition,all the four kinds of oil did not affect the cellulolytic bacteria such as Fibrobacter succinogens,Ruminococcus albus,and Ruminococcus flavefaciens;however,the oil supplementation increased Butyrivibrio fibrisolvens,and the promoting effects increased with unsaturated degree of oils.And generally,oil addition didn＇t significantly affect cellulose degradability（P0.05）.In conclusion,excepted for rapeseed oil,4% oil in the substrate had not significant influence on the cellulose degradability,while had profound effects on microbial population.