具有单一热源的对流双温PCR微流控装置的研究

Study of a convective two-temperature PCR microfluidics device with a single heat source

为了克服传统连续流动聚合酶链式反应(PCR)微系统经常需要多个加热源来获取PCR所需要的温度,且集成或者非集成的泵系统也需要用来驱动PCR溶液连续流动,从而实现PCR扩增的缺点,研究了一种具有单一热源的对流双温PCR微系统。通过复合肋片技术在沟槽铜片上形成对流双温PCR所需要的温度,由流体密度差形成的自然对流来驱动闭环通道中连续流动PCR。实验表明:开发的微流控PCR能在45 min内扩增112 bp的大肠杆菌DNA片段。

To overcome shortcomings of the conventional continuous-flow PCR microsystems usually need multiple heat sources to provide temperatures for PCR,and the integrated or nonintegrated pumping systems are also required to drive the PCR solution to flow continuously,and thus the PCR is realized.A convective two-temperature PCR microfluidics with a single heat source is studied.The temperatures for this PCR microsystem are formed on the grooved copper flake by the combined fin technology.And,the large temperature differences needed for PCR induce the differences in the flow density,which under certain conditions can be used to transport the PCR solution within a closed-loop channel.The experiment demonstrates that the developed microfluidic PCR can amplify the 112 bp Escherichia coli DNA fragment in 45min.