三七总皂苷对缺氧再给氧血管内皮细胞钙离子浓度及肌浆网Ca^(2+)-ATP酶活性的影响

The Effect of Total Panax Notoginseng Saponins on Calcium Ion and Sarcoplasmic Reticulum Ca^(2+)-ATP Ase of ECV304 in Hypoxia/reoxygenation Injury

目的:探讨人脐静脉内皮细胞株(ECV304)缺氧再给氧(H/R)损伤的发生机制及三七总皂苷(tPNS)对该病理生理过程的影响。方法:将体外培养的ECV304分为七组,正常组;模型组;tPNS高、中、低浓度组;维拉帕米组;吡咯烷二硫氨基甲酸酯(PDTC)组,对各组进行相应处理后,分别检测各组细胞内钙离子浓度[Ca2+]i、肌浆网Ca2+-ATP酶活性、一氧化氮(NO)及前列环素(PGI2)含量、细胞活力的变化。结果:模型组、tPNS高、中、低浓度组、维拉帕米组及PDTC组肌浆网Ca2+-ATP酶活性、一氧化氮(NO)及前列环素(PGI2)含量、细胞活力明显低于正常组,[Ca2+]i明显高于正常组,其中tPNS高、中、低浓度组、维拉帕米组及PDTC组肌浆网Ca2+-ATP酶活性、一氧化氮(NO)及前列环素(PGI2)含量、细胞活力明显高于H/R组,[Ca2+]i明显低于H/R组,且呈浓度依赖性改变,tPNS高浓度组肌浆网Ca2+-ATP酶活性、一氧化氮(NO)及前列环素(PGI2)含量、细胞活力明显高于维拉帕米组及PDTC组,[Ca2+]i明显低于维拉帕米组及PDTC组。结论:H/R可激活ECV304,使细胞肌浆网Ca2+-ATP酶活性、一氧化氮(NO)及前列环素(PGI2)含量、细胞活力明显降低,[Ca2+]i明显升高;tPNS能有效改善肌浆网Ca2+-ATP酶活性,减轻细胞内钙超载,减轻H/R损伤,且高浓度tPNS效果优于维拉帕米、PDTC。

Objective：To study the mechanisms of ECV304 with hypoxia/reo-xygenation（H/R） injury and the effect of total panax notoginseng saponins（tPNS） on the pathophysiologic process.Methods：Divided the culture ECV304 to 7 groups,control group;H/R group;high,middle and low concentrations of tPNS group;Verapamil group;PDTC group.Every group are taked corresponding disposition.The ECV304 i,sarcoplasmic reticulum Ca2＋-ATP ase were detected,The content of NO and PGI2,Cell viability were measured.Result：The cell of sarcoplasmic reticulum Ca2＋-ATP ase,content of NO and PGI2,Cell viability in H/R group,high,middle and low concentrations of tPNS group,Verapamil group,and PDTC group were significantly lower than that in control group.The levels of i in H/R group,high,middle and low concentrations of tPNS group,Verapamil group,and PDTC group were significantly higher than that in control group,but the cell of sarcoplasmic reticulum Ca2＋-ATP ase,content of NO and PGI2,Cell viability in high,middle and low concentrations of tPNS group,Verapamil group,and PDTC group were significantly higher and the levels of i Was significantly lower than that in H/R group（P0.05）,The higher concentration of tPNS is more effective.The high concentrations of tPNS group cell of sarcoplasmic reticulum Ca2＋-ATP ase,content of NO and PGI2,Cell viability is significantly higher than that in Verapamil group and PDTC group,the levels of i is significantly lower than that in Verapamil group and PDTC group.Conclusion： It seems that H/R activate ECV304,decreace the vitality of sarcoplasmic reticulum Ca2＋-ATP ase,content of NO and PGI2,Cell viability,increace i,and injury ECV304 function.The tPNS can increace the vitality of sarcoplasmic reticulum Ca2＋-ATP ase efficiently,decreace i and alleviate H/R injury,but also the high concentrations of tPNS group is more efficiently than Verapamil group and PDTC group.