摘要
目的:探讨超微血府逐瘀汤对急性心肌缺血大鼠缺血心肌血管新生及bFGF、ANG-1表达的影响。方法:采用结扎冠状动脉左前降支的方法复制急性心肌缺血模型,应用免疫组化法测定缺血心肌内皮细胞数和微血管密度(MVD),检测缺血心肌局部碱性成纤维细胞生长因子(bFGF)、血管紧张素1(ANG-1)表达的影响。结果:超微血府逐瘀汤组内皮细胞数、微血管密度及bFGF、ANG-1蛋白表达均有明显升高,与麝香保心丸组比较,差异显著(P<0.01或P<0.05)。结论:超微血府逐瘀汤能够促进心肌缺血大鼠缺血心肌血管新生,对缺血心肌具有保护作用,其作用机制可能与上调心肌局部bFGF、ANG-1蛋白表达有关。
Objective:To explore the effects of the Angiogenesis and the bFGF and ANG-1 protein expressions of the icro-pieces Xuefu Zhuyu decoction on rats with acute myocardial Ischemia.Methods:The left anterior descending coronary artery was ligated to make a model of acute ischemic myocardium.The microvessel vascular density(MVD) and the expression of bFGF and ANG-1 at protein level were observed in immuneohistochemical method.Results:Compared to Shexiang Baoxin group,the icro-pieces of Xuefu Zhuyu decoction could significantly enhance the levels of MVD and the protein expression of bFGF and ANG-1(P0.01 or P0.05).Conclusions:The icro-pieces of Xuefu Zhuyu Decoction could promote angiogenesis and upregulate the expression of bFGF and ANG-1.It has protective effects on acute myocardial ischemia in rats.Upregulating the expression of bFGF and ANG-1 may be one of its mechanisms.
出处
《中华中医药学刊》
CAS
2011年第4期773-775,共3页
Chinese Archives of Traditional Chinese Medicine
基金
湖南省教育厅青年基金资助项目(06B071)
湖南省自然科学基金资助项目(08JJ3056)