荷叶水提物抑制氧化低密度脂蛋白诱导的人脐静脉内皮细胞表达单核细胞趋化蛋白1和血管细胞黏附分子1

Water Extract of The Lotus Leaf Suppressed Expression of Monocyte Chemoattractant Protein-1(MCP-1)and Vascular Cell Adhesion Molecule 1(VCAM-1)Induced By Oxidized Low Density Lipoprotein(Ox-LDL)in Human Umbilical Vein Endothelial Cells

目的:探讨荷叶水提物对氧化低密度脂蛋白(Ox-LDL)诱导的人脐静脉内皮细胞(HUVECs)表达单核细胞趋化蛋白1(MCP-1)和血管细胞黏附分子1(VCAM-1)的影响。方法:(1)分别用25mg/L、50mg/L、100mg/L的Ox-LDL刺激培养的HUVECs 24h,半定量逆转录聚合酶链反应(RT-PCR)方法测定MCP-1和VCAM-1的mRNA表达,酶联免疫吸附法(ELISA)法测定细胞上清中MCP-1和VCAM-1的含量;(2)预先分别用10mg/L、20mg/L、40mg/L的荷叶水提物干预HUVECs,再用50mg/L的Ox-LDL刺激,半定量RT-PCR方法测定MCP-1和VCAM-1的mRNA表达,ELISA法测定细胞上清中MCP-1和VCAM-1的含量。结果:不同浓度的Ox-LDL刺激HUVECs后,MCP-1和VCAM-1 mRNA的表达及细胞上清中的含量呈浓度依赖性上调(P<0.01),且不同浓度的Ox-LDL组间差异亦有统计学意义(P<0.01)。荷叶水提物干预后,HUVECs的MCP-1 mRNA和VCAM-1 mRNA的表达及细胞上清MCP-1和VCAM-1含量呈浓度依赖性下降。结论:荷叶水提物能够抑制Ox-LDL诱导的HUVECs表达MCP-1和VCAM-1,可能通过这一机制有抗动脉粥样硬化的潜在作用。

Objective：To investigate the expressions of monocyte chemoattractant protein-1（MCP-1） and vascular cell adhesion molecule 1（VCAM-1） induced by oxidized low-density lipoprotein（Ox-LDL） in human umbilical vein endothelial cells（HUVECs） suppressed by water extract of the lotus leaf.Methods：HUVECs were cultured and treated with Ox-LDL of 3 different concentrations（25 microg/L,50 microg/L,and 100 microg/L） for 24 hours.We determined mRNA expressions of MCP-1 and VCAM-1 by semi-quantitative reverse-transcription polymerase chain reaction（RT-PCR）.Concentration of MCP-1 and VCAM-1 protein in medium was measured using the enzyme linked-immuno-sorbent assay（ELISA） method.Pretreated HUVECs with water extract of the lotus leaf 24 hours before adding Ox-LDL,then cells were treated with Ox-LDL（50 microg/L） for 24 hours.mRNA expressions of MCP-1 and VCAM-1 were determined by semi-quantitative RT-PCR.Concentration of MCP-1 and VCAM-1 protein in medium was measured using the ELISA method.Results：Exposure of HUVECs to Ox-LDL（25 microg /L,50 microg /L and 100 microg /L respectively） resulted in upregulation of MCP-1 and VCAM-1,both mRNA and protein expressions,in a dose-dependent manner.Expressions of MCP-1 and VCAM-1 induced by Ox-LDL（50 microg /L） were significantly suppressed by water extract of the lotus leaf in a dose-dependent manner.Conclusions：our results suggest that Ox-LDL can induce expressions of MCP-1 and VCAM-1 in a dose-dependent manner in HUVECs,and this process can be suppressed by water extract of the lotus leaf.