植物胰岛素的分离纯化及降糖作用的实验动物研究

Seperation and Purification of Plant Insulin and Its Hypoglycemic Effect on the Experimental Animals

目的:探寻大量纯化苦瓜中植物胰岛素的技术方法。方法:采用盐析、阴离子交换层析和凝胶过滤层析三步技术纯化苦瓜中植物胰岛素,并行电泳、层析和动物实验鉴定,壳聚糖包封。结果:目的提取物于11000处出现一条电泳带,相对迁移率与胰岛素相同;壳聚糖包封后苦瓜中植物胰岛素的降糖作用更显著。结论:本方法可适用于大量纯化苦瓜中植物胰岛素。

Objective： To explore a technical method to purify the plant insulin in the bitter melon.Methods：applying three-step methods to purify insulin from bitter melon these are salting out,ion exchange chromatography and gel filtration chromatography.And than using electrophoresis chromatography,animal experiments and chitosan envelopment methods to identify it.Results：The extracts appeared electrophoresis band at the 11000 molecular weight,and had the same relative mobility with insulin.The plant insulin from the bitter melons has the hypoglycemic effect significantly after the chitosan envelopment methods.Conclusion：To stabilized extract the plant insulin from the bitter melon can depend on this method mentiond in this paper.