湘A1号、2号对免疫缺陷动物免疫保护作用的研究

The Researches of Immune Protection Effects of Xiang A NO 1,Xiang A NO 2 on the Immunodeficiency Animal

目的:探讨湘A 1号、2号对免疫缺陷动物的免疫保护作用。方法:通过小鼠腹腔注射环孢菌素A建立免疫缺陷动物模型,观察湘A1号、2号对免疫缺陷动物模型胸腺重量指数、肺重量指数、肝脏重量指数、腹腔巨噬细胞吞噬功能以及胸腺、脾脏、肺和小肠组织的病理变化的影响。结果:与模型对照组比较,湘A1号的低、中、高剂量组及湘A2号中、高剂量组可显著提高胸腺重量指数(P<0.01或P<0.05),湘A1号高剂量组、湘A2号中、高剂量组能显著降低肺重量指数(P<0.01),湘A1号和A2号的低、中、高剂量组均可显著降低肝重量指数(P<0.01或P<0.05);湘A1号、湘A2号低、中、高剂量组能显著提高腹腔巨噬细胞吞噬百分率(P<0.01),湘A1号中、高剂量及湘A2号低、中、高剂量组能显著提高腹腔巨噬细胞的吞噬指数(P<0.01或P<0.05);湘A1号高剂量及湘A2号中剂量能明显减轻胸腺组织萎缩、局部脂肪变性,肺泡壁增厚,小肠上皮脱落等病理变化。结论:湘A1号、2号对环孢菌素A所致的免疫缺陷动物模型有一定的免疫保护作用。

Objective： To investigate the immune protection effect of Xiang A NO 1 and Xiang A NO 2 on the immunodeficiency animal.Methods： The immunodeficiency mice model is established by intraperitoneal injection of cyclosporine A.To observe the effects of Xiang A NO 1 and Xiang A NO 2 on thymus weight index,lung weight index,liver mass index,abdominal macrophage function as well as the pathological changes of the thymus,lungs,liver and the small intestine of Immunodeficiency mice.Results： Compared with the model group,The low-dose group,middle-dose group,high-dose group of Xiang A NO1and the middle-dose group,high-dose group of Xiang A NO 2 could significantly enhance the immune organs thymus mass index（P0.01 or P0.05）;The high-dose group of Xiang A NO1 and the middle-dose group,high-dose group of Xiang A NO 2 could significantly reduce lung weight index（P0.01）;The low-dose group,middle-dose group,high-dose group of Xiang A NO1 and Xiang A NO 2 could significantly reduce liver mass index（P0.01 or P0.05）;The low-dose group,middle-dose group,high-dose group of Xiang A NO1 and Xiang A NO 2 could significantly enhance the phagocytic percentage of peritoneal macrophages（P0.01）;The middle-dose group,high-dose group of Xiang A NO1 and the low-dose group,middle-dose group,high-dose group of Xiang A NO 2 could significantly enhance the phagocytic index of peritoneal macrophages（P0.01 or P0.05）;The high-dore group of Xiang NO1 and the middle-dnse group of Xiang A NO 2 bould rignificantlx reduce qatholngical changes of thd thxmts atrnphy,partial fatty degeneration,alveolar wall thickening and Intestinal epithelial shedding.Conclusion： Xiang A NO1 and Xiang A NO2 play Immune protection On immunodeficiency mice model established by intraperitoneal injection of cyclosporine A.