自发性糖尿病小鼠C57BL/KsJ-db/db某些脏器组织病理学观察

Pathological Changes of Some Organs in Spontaneous Diabetes C57BL/KsJ-db/db Mice

目的观察自发性糖尿病C57BL/KsJ-db/db小鼠胰腺、肾脏、睾丸和卵巢的常规病理变化。方法取血糖高于10.1 mmol/L的2、7、12月龄db/db纯合仔小鼠,摘眼球放血后取胰腺、肾、睾丸、卵巢放入10%福尔马林溶液中,HE染色,常规病理切片。结果 7、12月龄胰腺见淋巴细胞浸润,部分胰岛体积较大,小导管样病变有增生扩张及潴溜;小鼠肾脏见部分肾小球细胞数增多,系膜细胞增生,系膜基质增多;卵巢较小,卵泡较少,未见黄体;睾丸生精功能弱。结论 C57BL/KsJ-db/db小鼠的自发性糖尿病会在一定程度上出现胰腺、肾脏、睾丸和卵巢等脏器发生病理改变。

Objective To observe routine pathological changes of the pancreas,kidney,testis and ovary in spontaneous diabetic mice.Methods C57BL /KsJ-db /db mice with blood glucose over 10.1 mmol /L at 2,7 and 12 month entered this study,after sacrificed,their organs pancreas,kidney,testis and ovary were soaked in 10% formalin for HE coloration and general pathological section.Results At 7 or 12 month,the pancreas tissues had lymphocyte infiltration,partial pancreas islets size increase and condulet hyperplasy ectasia;the kidney showed partial glomerular cell numbers increase,mesangial cell hyperplasy and basilar membrane thickening;the ovary was small with less egg follicle and missing corepus luteum;the testis was weak in spermatozoa genesis.Conclusion Some pathological changes of pancreas,kidney,testis and ovary may be observed in the C57BL /KsJ-db /db mice of spontaneous diabetes.