卷叶贝母多倍体鳞茎诱导方法研究

Study on the Induction of Polyploid Bulb of Fritillaria Cirrhosa D. Don

[目的]利用组织培养技术快速诱导生成卷叶贝母(Fritillaria cirrhosaD.Don)多倍体鳞茎。[方法]用一定浓度的秋水仙素溶液浸泡经预分化培养的卷叶贝母紧密愈伤组织团块,再经过诱导分化培养获得多倍体鳞茎。[结果]用浓度为1.2 g/L的秋水仙素溶液浸泡处理卷叶贝母紧密愈伤组织团块24 h后,接种在MS+6-BA 1.0 mg/L+NAA 0.3 mg/L的分化培养基上培养,其多倍体鳞茎的诱导率最高为83.3%,细胞染色体数为2n=4x=48,总生物碱含量为0.249%。[结论]用组织培养技术获得优质高产卷叶贝母多倍体鳞茎的方法是可行的。

[Objective] Rolyploid bulb of Fritillaria cirrhosa D.Don is introduced by tissue culture technique.[Methods] The compact callus cell mass of Fritillaria cirrhosa D.Don which are differentiation cultured beforehand are soaked in colchicine solution,and then cultured in the differential medium of MS＋6-BA 1.0 mg/L＋NAA 0.3 mg/L.Polyploid bulb are obtained by induction differentiation culture.[Result] Colchicine solution of 1.2 g/L is used to douse the compact callus cell mass of Fritillaria cirrhosa D.Don for 24 h.The highest inductivity of polyploid bulb was 83.3%.[Conclusion] Chromosome number is 2n=4x=48 and the total alkaloids content is detected to be 0.249%.It indicates that it is feasible to obtain high quality and high yield of Fritillaria cirrhosa D.Don polyploid bulb by tissue culture technique.