基于丝网印刷碳电极的微囊藻毒素-(亮氨酸-精氨酸)的电流型免疫传感器

An Amperometric Immunosensor for Microcystin-(Leucine-Arginine) Based on Screen-Printed Carbon Electrode

将微囊藻毒素-（亮氨酸-精氨酸）-鸡卵白蛋白（Microcystin-（leucine-arginine）-ovalbumin,MCLR-OVA）固定在锇联吡啶聚（4-乙烯基吡啶）聚合物修饰的丝网印刷碳电极表面,制备了一种检测MCLR的电流型免疫传感器。该传感器基于间接竞争免疫分析模式,以辣根过氧化物酶偶联的羊抗鼠免疫球蛋白抗体为标记物。其电流信号响应与MCLR的浓度在0.43~10.72 mg/L范围内呈负线性相关,检出限为0.17 mg/L。3种实际水样的平均添加回收率为83%~121%,相对标准偏差为3.6%~7.6%。本方法为MCLR的环境监测提供了科学依据。

An amperometric immunosensor for the detection of microcystin-（leucine-arginine）（MCLR） was prepared by immobilizing an MCLR-ovalbumin（MCLR-OVA） conjugate to an osmium bipyridyl complex-attached poly（4-vinylpyridine） membrane modified onto a screen-printed carbon electrode.The immunosensor was based on the indirect competitive immunoassay format with the labeled agent of horseradish peroxidase-conjugated goat anti-mouse immunoglobulin-G antibody.The current response decreased proportionally with the concentration of MCLR in the range of 0.43-10.72 mg/L with a detection limit of 0.17 mg/L.The mean recoveries of standard additions in three real water samples were in the range of 83% to 121%,and the RSD were in the range of 3.6% to 7.6%.Thus,the proposed method is a promising technique for the detection of MCLR in environmental monitoring.