摘要
试验从肠炎沙门菌国内标准株50336中抽提和纯化SEF14菌毛,并制备SEF14蛋白鼠源高免血清。结果发现,肠炎沙门菌在CFA培养液中,静止培养50h左右,SEF14菌毛表达量相对较多,在机械高速匀浆后,经进一步的透析纯化,得到较纯的SEF14菌毛蛋白,蛋白大小和相关报道一致,约14ku左右。Western-blotting检测发现纯化的重组蛋白rSEFA(体外表达的SEF14菌毛主要亚单位蛋白SEFA)免疫小鼠得到的高免血清能识别标准株肠炎沙门菌SEF14菌毛蛋白以及纯化的重组蛋白rSEFA,说明体外抽提的SEF14菌毛蛋白和体外表达的rSEFA蛋白同样具有较好的免疫原性和反应原性。
SEF14 fimbriae were extracted and purificated from S.enteritidis reference strain 50336,and high titer of anti-SEF14 mouse sera was made in this. The results showed that S.enteritidis could express SEF14 fimbriae by caltured in CFA medium statically in vitro. And SEF14 fimbriae was separated from S.enteritidis at room temperature by shearing and dialyzing,which of molecular weight was about 14 ku. High titer mouse sera of anti-rSEFA （recombinant subunit protein SEFA expressed in vitro） was prepared and detected by Western-blotting,and the mouse sera could recognize the purified SEF14 fimbriae from reference strain 50336 and rSEFA,respectively. This indicated that SEF14 fimbriae had good immunogenicity and reactogenicity.
出处
《中国家禽》
北大核心
2011年第7期19-22,共4页
China Poultry
基金
国家自然科学基金(30571374
30771603)
江苏省属高校自然科学重大基础研究项目(08KJA230002)
