益气化痰方药对哮喘小鼠气道杯状细胞及muc5ac的影响

Effects of complex prescriptions for replenishing qi and resolving phlegm on goblet cell and muc5ac of asthma mice airway

目的探讨益气化痰方药对哮喘小鼠气道粘液高分泌的影响及其机制。方法 BALB/c小鼠随机分为正常对照组、哮喘组、益气化痰方药组和醋酸波尼松组,各组肺组织苏木素-伊红(HE)染色观察气道炎症,应用过碘酸雪夫氏染色(PAS)观察气道杯状细胞变化,应用ELISA法检测肺组织匀浆黏蛋白(muc5 ac)表达的变化。结果哮喘组与正常对照组相比,气道炎性细胞浸润、粘膜增生、杯状细胞增生、muc5 ac蛋白表达水平增高,而益气化痰方药组和醋酸波尼松组上述表现明显较哮喘组减轻。结论益气化痰方药能改善哮喘气道炎症,减少杯状细胞增生及muc5 ac表达。

Objective To investigate the effects of complex prescriptions for replenishing qi and resolving phlegm on asthma airway mucus hypersecretion and its mechanism. Methods BALB/c mice were randomly divided into normal control group, asthma group, prednisone acetate group and complex prescriptions group, pulmonary histological changes were assessed by the lung tissue hematoxylin-eo- sin （HE） staining and airway goblet cell changes by periodic acid Schiff （PAS） staining, Additionally, the lung muein （muc5ae） expres- sion was determined by ELISA. Results In the asthma group, inflammatory cells, mucosal hyperplasia and airway goblet cell hyperplasia and the the level of mucSac protein in lung was significantly higher than the control group. In comparison, in they were significantly reduced prednisone acetate group and complex prescriptions group. Conclusion complex prescriptions for replenishing qi and resolving phlegm can improve airway inflammation and reduce goblet cell hyperplasia and muc5ac expression.